Day-night rhythm in human skeletal muscle metabolism

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 26

Inclusion Criteria

• Caucasian
• Healthy (as determined by dependent physician based on medical questionnaire)
• Male
• Age: 18-35 years
• Normal BMI (18-25 kg/m2)
• Regular sleeping time (normally 7-9h daily)

Exclusion Criteria

• Extreme early bird or extreme night person (score <=30 or >=70 on MEQ-SA questionnaire)
• Heavily varying sleep-wake rhythm
• Shiftwork during last 3 months
• Travel across >1 time zone in the last 3 months
• Engagement in exercise > 2 hours total per week
• Using >400mg caffeine daily (more than 4 coffee or energy drink)
• Smoking
• Unstable body weight (weight gain or loss > 3kg in the last 3 months)
• Significant food allergies/intolerance (seriously hampering study meals)
• Participation in another biomedical study within 1 month before the first study visit;• Any contra-indication to Magnetic Resonance Imaging (MRI) scanning:
o Any implant or prosthesis that is contra-indicated for MRI
o Metal containing foreign bodies in the eye or brain
o Any other metal containing objects contra-indicated for MRI scanning
o Claustrophobia
o Epilepsy;• Any contra-indication to the CorTemp telemetric pill:
o Known or suspected obstructive disease of the gastrointestinal tract
o History of disorders or impairment of the gag reflex
o Previous gastrointestinal surgery
o Felinization of the esophagus
o Hypomotility disorders of the gastro-intestinal tract
o Implanted electromedical device;• Previous allergic reaction to Tegaderm hypoallergenic medical adhesive covering.
• Any acute condition, exacerbation of chronic condition, or medical history that would in the investigator*s opinion interfere with the study

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main end-point is a day-night variation/rhythm in skeletal muscle<br /><br>mitochondrial function. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary study parameters are day-night variations/rhythms in whole-body<br /><br>energy metabolism, skeletal muscle mRNA and protein levels and liver<br /><br>triglyceride concentration.</p><br>