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Evaluation of Survival of Mature Second Premolar Treated With Regenerative Techniques

Not Applicable
Conditions
Carious Teeth
Interventions
Biological: PRP
Biological: PRF
Registration Number
NCT02915419
Lead Sponsor
Cairo University
Brief Summary

This study aims to compare the ability of different regenerative protocols in the treatment of mature necrotic

Detailed Description

American Dental Association adopted the tissue engineering concept and pulp regeneration in 2009. Regeneration was focused on the treatment of immature necrotic teeth so as to allow root completion, improving both the functionality and durability of the affected tooth. Utilizing a blood clot in the affected tooth with immature roots was very beneficial, as it acts as a scaffold for the migration of stem cells and morphogens to allow regeneration .

Based on the success of pulp regeneration in treating immature teeth, ambitious dentists started to look forward on regenerating the pulp of mature teeth as a substitution to the conventional root canal treatment. On the other hand, lots of clinicians were very doubtful about treating mature teeth with the same protocol, as the small apical foramen might not provide a good portal for the entry of stem cells and growth factors, which in turn is very important for the success of pulp regeneration.Doubts in treating mature apex encouraged researchers to look for an alternative to the stem cells other than the apical papilla, from this concept the idea for using an alternative source of stem cells and growth factors as platelet rich plasma (PRP) emerged. The use of platelet rich plasma (PRP) as a potentially ideal scaffold for regenerative endodontic therapy has been reported in the treatment of immature teeth. However, the use of bovine thrombin for the activation of PRP has been an issue of controversy, as it requires non autologous anticoagulant known to hinder the process of pulp regeneration.

This led to the development of the second generation, platelet concentrate known as Choukroun's platelet rich fibrin (PRF) which is totally autologous in nature. Platelet rich fibrin (PRF) was very promising, but it is debatable whether to use PRP or PRF.The objective of this study is to evaluate novel regenerative techniques, utilizing platelet rich plasma and platelet rich fibrin in treatment of second premolar teeth with necrotic pulp, mature apex, and periapical lesion. This is a trial to prolong the survival of the affected tooth, and to aid in complete healing of periapical lesion, and to regain pulp vitality and sensitivity.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Patients who are free from any physical or mental handicapping condition with no underlying systemic diseases.

Non-pregnant females Single canalled second premolar

  • Patients having non-vital, mature, second premolar teeth, radiographic evidence of periapical lesion.
  • Positive patient/Guardian compliance for participation in the study.
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Exclusion Criteria
  • Any known sensitivity or adverse reactions to medicaments or pharmaceuticals necessary to complete the trial.

Non-restorable coronal portion of teeth involved in the trial. Vital teeth Immature teeth Traumatized teeth Radiographic evidence of external or internal root resorption. Any criterion, not mentioned in the inclusion criteria

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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
group APRPprp revascularization
group APRFprp revascularization
group BPRPtreatment of affected teeth using platelet rich fibrin by applying prf in steril root canal
group BPRFtreatment of affected teeth using platelet rich fibrin by applying prf in steril root canal
Primary Outcome Measures
NameTimeMethod
Survival1 year

how long will the tooth survive after treatment, testing the durability of tooth

Secondary Outcome Measures
NameTimeMethod
healing1 year

measuring the density of lesion using the cone beam CT

vitality1 year

after how many days will the tooth respond normaly to stimulus

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