Treatment of gum deficiency using two layered membrane prepared from blood

Completed

• Conditions: Gingival recession,

• Registration Number: CTRI/2022/07/044099
• Lead Sponsor: Anupama Tadepalli

Brief Summary

Gingival recession is described as apical migration of the free gingival margin relative to the cemento enamel junction leading to root exposure. The prevalence of mid-buccal recession defects was 91.6% as indicated in a recent epidemiological study conducted by NHANES 2020.Gingival recession is often associated with dentinal hypersensitivity, esthetic issues and also root caries. Periodontal plastic procedures aim to achieve complete root coverage while enhancing aesthetics by integrating mucosa and keratinized gingiva with adjacent tissues.

Periodontal wound healing is a complex biological mechanism where multiple cellular events occur concurrently leading to repair or regeneration.Platelets are the initial cells involved in the wound healing process. Upon activation, they release various bioactive molecules, cytokines, and adhesive proteins stimulating inflammatory cells to populate in the surgical area thereby setting the pace for the wound healing process.

A-PRF obtained by centrifugation protocol of 1500rpm for 14min at 200G, exhibited porous fibrin structure with larger interfibrinous space incorporating quantitatively more platelets and inflammatory cells, evenly distributed all through the matrix releasing significantly increased growth factor proteins (VEGF, PDGF, TGF-β, IGF and EGF). A-PRF was found to contain increased levels of VEGF, responsible for neoangiogenesis, monocytes as a source for BMP’s and fibronectin for extracellular matrix formation. The potential benefit of multilayer advanced platelet rich fibrin in management of gingival recession has not yet been established. The aim of this clinical study was to evaluate and compare the clinical efficacy of conventional gold standard connective tissue graft and single/ bi layered A-PRF in the treatment of gingival recession defects.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-07-25
• Study Completion: 2023-03-07
• Last Update Posted: 2023-06-07

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 45

Inclusion Criteria

• Patients with Cairo’s RT1 & RT2 recession defects in maxillary anteriors and premolars 2.
• Minimum recession depth ≥ 2mm.

Exclusion Criteria

• Recession sites with probing depth ≥ 4mm and mobile teeth 2.
• Teeth with prosthetic restorations 3.
• Anemic patients 4.
• Individuals with Platelet disorders 5.
• Individuals with infectious diseases 6.
• Patients with poor oral hygiene 7.
• Systemic condition /disease/any medication influencing the periodontal therapy and disease.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
1.To assess and compare the changes in Recession height (RH) between the study groups at baseline and 6 months follow up.	Baseline, 3 months and 6 months
2. To compare the mean Root coverage% (RC%) between the study groups at 6 months follow up.	Baseline, 3 months and 6 months

Secondary Outcome Measures

Name	Time	Method
1. To evaluate the comparative changes in Gingival thickness (GT) between the study groups at baseline and 6 months follow up.	2. To evaluate the comparative changes in Width of keratinized tissue (WKT) between the study groups at baseline and 6 months follow up

Trial Locations

Locations (1)

SRM Dental College and Hospital; 🇮🇳 Chennai, TAMIL NADU, India

SRM Dental College and Hospital

🇮🇳Chennai, TAMIL NADU, India

DrAnupama Tadepalli

Principal investigator

09677065517

anupamamds@gmail.com