Research on Precise Immune Prevention and Treatment of Glioma Based on Multi-omics Sequencing Data
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- expression level of low molecular weight protein，drived from Metabonomics
研究概览
简要总结
This project intends to use multiple types of biological samples from glioma patients and mouse intracranial tumor models as research objects, and comprehensively apply a series of omics sequencing technologies and molecular biology technologies to jointly define the following research objectives :
详细描述
- Through high-throughput sequencing technologies such as， genome sequencing, transcriptome sequencing and single-cell sequencing, Proteomics，Metabonomics and Metagenomics， the similarities and differences of the glioma immune microenvironment within and between individuals were revealed from the multi-dimensional and big data level, and the immune molecular typing of glioma was established.
- Establish and verify that the neoantigen polypeptides predicted by AI algorithm after analyzing multi-omics data (genome, transcriptome, etc.) have good killing effect on individual tumor cells.
- To establish an intelligent evaluation model of glioma immunotyping by integrating imaging, pathology, high-throughput sequencing data and other omics information, and to provide a visual tool for monitoring changes in the intratumoral microenvironment of glioma patients, with the expectation of helping timely intervention of immunotherapy in patients with recurrence.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Other
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •The patients with glioma in the Department of Neurosurgery of Huashan Hospital Affiliated to Fudan University who meet the following three conditions can be enrolled
- •They were 18-80 years old, male and female;
- •The pathological diagnosis was glioma;
- •On the basis of not affecting the clinical routine diagnosis, tissue (6 mm * 6 mm) or paraffin section (5 pieces) can be used for research;
- •Sign informed consent or ethics committee approval
排除标准
- 未提供
结局指标
主要结局
expression level of low molecular weight protein，drived from Metabonomics
时间窗: 24 month
the expression level and interaction of different Metabolites，including D-2-Hydroxyglutarate. etc.
Reads Per Kilobase Million（RPKM），KB^(-1), drived from Transcriptome sequencing
时间窗: 24 month
the expression level of RNA,including mRNA、 miRNA、circRNA.etc
expression level of DNA，drived from Metagenomics
时间窗: 24 month
Differences about DNA expression of all microorganisms in the environment
Overall Survival（OS），month
时间窗: 24 month
Survival time from surgery to death in 120 patients with glioma.
expression level of protein，drived from paraffin-embedded specimens， retrospectively collected.
时间窗: 24month
different expression level of proteins in Gliomas with different grades and molecular subgroups (1000 cases)
Progress Free Survival （PFS），month
时间窗: 24 month
Survival time from surgery to first progression or death from any cause in patients with glioma.
Tumor Mutation Burden（TMB），mutations/mb, drived from Genomic sequencing
时间窗: 24 month
the level of gene mutation，including TERT、GMEM、IDH.etc.
expression level of protein , drived from Protein sequencing
时间窗: 24 month
the expression level of protein, post-translational modification and protein-protein interaction，including PD1、PDL1、CD4、CD8.etc.
frequency of TCR/BCR clone，drived from Immunomics
时间窗: 24month
different expression level and interaction of Immune molecules，including TCR、BCR、antibody.etc.
次要结局
未报告次要终点
研究者
Yu Yao, MD
Professor
Huashan Hospital