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A Study of Valcyte (Valganciclovir) CMV Prophylaxis After Renal Transplantation

Registration Number
NCT00372229
Lead Sponsor
Hoffmann-La Roche
Brief Summary

This 2 arm study will compare the efficacy of 100 days of Valcyte (900mg po daily) prophylaxis with that of no prophylaxis, under the condition of pre-emptive therapy of active CMV infection, in CMV positive renal transplant recipients. The influence of the two prevention concepts on the occurrence of direct and indirect effects of active CMV infections will be compared. The anticipated time on study treatment is 3 months-1 year, and the target sample size is 100-500 individuals.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
299
Inclusion Criteria
  • primary or secondary renal allograft within preceding 14 days;
  • IgG seropositive for CMV;
  • receiving immunosuppressive therapy.
Exclusion Criteria
  • active CMV infection;
  • current/history of malignancy;
  • acute steroid resistant rejection episode since transplantation.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Pre-emptive CMV TherapyValganciclovir (Pre-emptive CMV Therapy)-
Valganciclovir Cytomegalovirus (CMV) ProphylaxisValganciclovir CMV Prophylaxis-
Pre-emptive CMV TherapyGanciclovir-
Primary Outcome Measures
NameTimeMethod
Urine Proteomic Pattern at Month 12Up to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. Urine proteomic pattern was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Percentage of Participants With Graft Loss at Month 84Up to 84 months
Percentage of Participants With Active Cytomegalovirus (CMV) Infection Within 12 MonthsUp to 12 months

Active CMV infection was defined as plasma polymerase chain reaction (PCR) ≥ 400 copies/millilitre (ml).

Percentage of Participants With CMV Disease Within 12 Months Including CMV Syndrome and Tissue Invasive DiseaseUp to 12 months

CMV disease comprises the two components of CMV syndrome as well as CMV tissue invasive disease. CMV syndrome was defined as viremia according to plasma PCR ≥ 400 copies/ml and at least one of the following signs: fever of ≥38 °C; new or increased malaise (malaise defined as normal activity reduced \>50%; cannot work or unable to care for self; leukopenia on 2 successive measurements separated by at least 24 hours thrombocytopenia; elevation of hepatic transaminases (alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to at least 2 x upper limit of normal (ULN). CMV tissue invasive disease was defined as viremia according plasma PCR ≥ 400 copies/ml and clinical evidence of localized CMV infection (CMV inclusion cells or in situ detection of CMV antigen or deoxyribonucleic acid \[DNA\] by immunostaining or hybridization, respectively), cerebral spinal fluid \[CSF\]) and/or relevant symptoms or signs of organ dysfunction.

Secondary Outcome Measures
NameTimeMethod
Percentage of Participants With Leukopenia Within 12 MonthsUp to 12 months

Leukopenia: white blood cell (WBC) of \< 3,500/microlitre (μL) and \< 1,000/μL

Percentage of Participants With Any Opportunistic Infection Within 12 MonthsUp to 12 months
Percentage of Participants With Post-Transplant Diabetes MellitusUp to 12 months
Percentage of Participants With Active CMV Infections Not Responding to Valganciclovir or IV Ganciclovir TreatmentUp to 12 months

Active CMV infection was defined as plasma polymerase chain reaction (PCR) ≥ 400 copies/millilitre (ml).

Percentage of Participants With Graft Survival at Month 12Up to 12 months
Number of Participants With CMV Viremia (Active CMV Infection) From Baseline to Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

Viremia (active CMV Infection) was defined as PCR ≥ 400 copies/ml.

Proteomics Parameter: CKD273Up to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. The proteomics of CKD273 was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Proteomics Parameter: CMVUp to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. The proteomics of CMV was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Proteomics Parameter: NephropathyUp to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. The proteomics of nephropathy was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Number of Participants With CMV Disease From Baseline to Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

CMV disease comprises the two components of CMV syndrome as well as CMV tissue invasive disease. CMV syndrome was defined as viremia according to plasma PCR ≥ 400 copies/ml and at least one of the following signs: fever of ≥38 °C; new or increased malaise (malaise defined as normal activity reduced \>50%; cannot work or unable to care for self; leukopenia on 2 successive measurements separated by at least 24 hours thrombocytopenia; elevation of hepatic transaminases (alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to at least 2 x upper limit of normal (ULN). CMV tissue invasive disease was defined as viremia according plasma PCR ≥ 400 copies/ml and clinical evidence of localized CMV infection (CMV inclusion cells or in situ detection of CMV antigen or deoxyribonucleic acid \[DNA\] by immunostaining or hybridization, respectively), cerebral spinal fluid \[CSF\]) and/or relevant symptoms or signs of organ dysfunction.

Time to Occurrence of First Viremia Within 12 MonthsUp to 12 months

Viremia was defined as plasma PCR ≥ 400 copies/ml.

Creatinine Clearance at Month 12Up to 12 months

Creatinine clearance was estimated using the Cockcroft-Gault formula.

Relationship Between Proteomics Pattern and Graft SurvivalUp to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. The proteomics of CKD273, CMV, and nephropathy was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Relationship Between Proteomics Pattern and Participant SurvivalUp to 12 months

Proteomics is the complete set of proteins expressed by an organism, tissue, or cell. The proteomics of CKD273, CMV, and nephropathy was measured on a scale between -1, indicating no graft alteration, and +1, indicating graft alteration.

Percentage of Participants Surviving at Month 12Up to 12 months
Percentage of Participants With Neutropenia Within 12 MonthsUp to 12 months

Neutropenia: absolute neutrophil count (ANC) \< 750/μL within 12 months.

Percentage of Participants With CMV Syndrome Within 12 MonthsUp to 12 months

CMV syndrome was defined as viremia according to plasma PCR ≥ 400 copies/ml and at least one of the following signs: fever of ≥38 °C; new or increased malaise (malaise defined as normal activity reduced \>50%; cannot work or unable to care for self; leukopenia on 2 successive measurements separated by at least 24 hours thrombocytopenia; elevation of hepatic transaminases (alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to at least 2 x upper limit of normal (ULN).

Percentage of Participants With CMV Tissue Invasive Disease Within 12 MonthsUp to 12 months

CMV tissue invasive disease was defined as viremia: PCR ≥ 400 copies/ml and clinical evidence of localized CMV infection (CMV inclusion cells or in situ detection of CMV antigen or deoxyribonucleic acid \[DNA\] by immunostaining or hybridization, respectively), cerebral spinal fluid \[CSF\]) and/or relevant symptoms or signs of organ dysfunction.

Viral Burden at ViremiaUp to 12 months

Time-weighted area under the curve (AUC) of the polymerase chain reaction (PCR). Viremia was defined as plasma PCR ≥ 400 copies/ml.

Days of HospitalizationUp to 12 months
Number of Participants With CMV Syndrome From Baseline to Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

CMV syndrome was defined as viremia according to plasma PCR ≥ 400 copies/ml and at least one of the following signs: fever of ≥38 °C; new or increased malaise (malaise defined as normal activity reduced \>50%; cannot work or unable to care for self; leukopenia on 2 successive measurements separated by at least 24 hours thrombocytopenia; elevation of hepatic transaminases (alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to at least 2 x upper limit of normal (ULN).

Percentage of Participants With at Least One Treated and Biopsy-Proven Acute Rejection Episode Within 12 MonthsUp to 12 months
Number of Participants With CMV Tissue Invasive Disease From Baseline to Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

CMV tissue invasive disease was defined as viremia according plasma PCR ≥ 400 copies/ml and clinical evidence of localized CMV infection (CMV inclusion cells or in situ detection of CMV antigen or deoxyribonucleic acid \[DNA\] by immunostaining or hybridization, respectively), cerebral spinal fluid \[CSF\]) and/or relevant symptoms or signs of organ dysfunction.

Percentage of Participants Surviving at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84
Number of Participants Who Died From Months 24 to Month 84From Month 24 to Month 84
Percentage of Participants With Graft Survival or Participant Survival at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84
Creatinine Clearance at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

Creatinine Clearance estimated by Cockcroft-Gault formula.

Number of Participants With Active CMV Infection After Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

Active CMV infection was defined as plasma polymerase chain reaction (PCR) ≥ 400 copies/millilitre (ml).

Number of Participants With Active CMV Infection Who Had Lost Their Transplant up to Month 84From Month 24 to Month 84
Kaplan-Meier Estimate of the Percentage of Participants (With Versus Without Active CMV Infection) on Pre-emptive CMV Therapy With First Occurrence of Graft Loss at Month 84Up to 84 months

An analysis for the time to first occurrence of graft loss within month 84 (by means of Kaplan-Meier analysis) was done for all patients who suffered at least once from acute CMV infection (CMV viremia) during this study versus all patients who did not suffer from acute CMV infection (CMV viremia) during the study. Active CMV infection was defined as plasma polymerase chain reaction (PCR) ≥ 400 copies/millilitre (ml).

Number of Participants With Graft Rejections by CMV Status (Positive or Negative) of the Donor at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84
Number of Participants With CMV Disease After Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84

CMV disease comprises the two components of CMV syndrome as well as CMV tissue invasive disease. CMV syndrome was defined as viremia according to plasma PCR ≥ 400 copies/ml and at least one of the following signs: fever of ≥38 °C; new or increased malaise (malaise defined as normal activity reduced \>50%; cannot work or unable to care for self; leukopenia on 2 successive measurements separated by at least 24 hours thrombocytopenia; elevation of hepatic transaminases (alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to at least 2 x upper limit of normal (ULN). CMV tissue invasive disease was defined as viremia according plasma PCR ≥ 400 copies/ml and clinical evidence of localized CMV infection (CMV inclusion cells or in situ detection of CMV antigen or deoxyribonucleic acid \[DNA\] by immunostaining or hybridization, respectively), cerebral spinal fluid \[CSF\]) and/or relevant symptoms or signs of organ dysfunction.

Percentage of Participants With Graft Survival at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84
Number of Participants Who Had Lost Their Transplant up to Month 84From Month 24 to Month 84
Number of Participants Without Active CMV Infection Who Had Lost Their Transplant up to Month 84From Month 24 to Month 84
Kaplan-Meier Estimate of the Percentage of Participants (With Versus Without Active CMV Infection) on Valganciclovir CMV Prophylaxis With First Occurrence of Graft Loss at Month 84Up to 84 months

An analysis for the time to first occurrence of graft loss within month 84 (by means of Kaplan-Meier analysis) was done for all patients who suffered at least once from acute CMV infection (CMV viremia) during this study versus all patients who did not suffer from acute CMV infection (CMV viremia) during the study. Active CMV infection was defined as plasma polymerase chain reaction (PCR) ≥ 400 copies/millilitre (ml).

Number of Participants Who Had Lost Their Transplant or Died up to Month 84From Month 24 to Month 84
Number of Graft Rejections by CMV Status (Positive or Negative) of the Donor at Month 24 and Every 12 Months up to Month 84From Month 24 to Month 84
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