Protein requirements in healthy adult men as assessed by the Indicator Amino Acid Oxidation (IAAO) technique: a replication study

Completed

Conditions: Eiwitbehoefte
Protein requirement

Registration Number: NL-OMON50939

Lead Sponsor: niversiteit Maastricht

Brief Summary: Trial is onging in other countries

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 12

Inclusion Criteria

- Male
- Aged between 18-35 years
- Healthy, recreationally active (exercise at least 1 per two weeks and maximum
4 days a week)
- 18.5 <= BMI <= 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with
daily living in an independent manner).

Exclusion Criteria

- Smoking
- Musculoskeletal disorders
- Metabolic disorders
- Use of any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories, or prescribed acne
medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The primary endpoint is the estimated average requirement (EAR) and recommended<br /><br>daily allowance (RDA) for dietary protein, which will be determined using the<br /><br>IAAO method by measuring 13C enrichment in expired breath and enrichment of<br /><br>L-[1-13C]-phenylalanine in plasma and urine.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>To determine carbon dioxide production, 13CO2 production, plasma and urine<br /><br>L-[1-13C]-phenylalanine enrichments, and plasma and urine phenylalanine<br /><br>concentration in a young, healthy, male population when applying the IAAO<br /><br>technique.</p><br>