Determination of protein profiles in vitreous of eyes with endophthalmitis with Surface Enhanced Laser desorption/ ionization time-of-flight (SELDI-tof) technology.
Completed
- Conditions
- Infection of vitreousinflammation of ocular fluid10021877
- Registration Number
- NL-OMON33140
- Lead Sponsor
- niversitair Medisch Centrum Utrecht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
Patients with endophthalmitis:
-
-Age >= 18 years
-initiation of endophthalmitis within 6 weeks after cataract surgery;Controll group:
-wils
- Age >= 18 years
-patient that undergo a pars plana vitrectomy because of a macular hole or
macular pucker.
Exclusion Criteria
Endophthalmitis group:
-patients able to give informed consent
-previous uveitis in the affected eye
-previous pars plana vitrectomy in the affected eye.;Controll group: macylar hle/ macular pucker
-retinopathy because of Diabetes Mellitus or previous uveitis in the affected eye
-previous pars plana vitrectomy in the affected eye.;-
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Three analyses will be performed with SELDI-tof in comparison to regular<br /><br>culture methods:<br /><br>1. Comparison of protein composition of vitreous in comparison to<br /><br>serum obtained bij surgery for maucular hole or<br /><br>macular pucker: controll population<br /><br>2. Comparison of protein composition of vitreous in comparison to<br /><br>serum of patients with endophthalmtis<br /><br>3. Comparison of protein composition of vitreous in comparison to<br /><br>serum of patients with endophthalmitis versus<br /><br>the controll population.</p><br>
- Secondary Outcome Measures
Name Time Method <p>1. Evaluation of the correlation between specific bacterial causes<br /><br>of endophthalmitis indentified by culture and<br /><br>specific protein pattern obtained via SELDI-tof analyses.<br /><br>2. Evaluation of the correlation of vitreous of endophthalmitis<br /><br>with a negative result with culture and the protein<br /><br>pattern obtained by SELDI-tof analyses. We will compare the<br /><br>protein patterns of culture negative results with<br /><br>peak pattern of bacteriae identified by culture.</p><br>