The peritoneal microvascular endothelial glycocalyx in peritoneal dialysis patients

Conditions: peritoneal dialysis
peritoneal vascular abnormalities
10029149
10003216

Registration Number: NL-OMON34683

Lead Sponsor: AMC

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: Not specified

Target Recruitment: 80

Inclusion Criteria

1. Patients with end stage renal disease starting peritoneal dialysis (PD) (at the moment of catheter implantation) or undergoing preemptive kidney transplantation;
2. Stable prevalent PD patients, free of acute inflammatory conditions and malignancy, undergoing any procedure involving the opening of the peritoneal cavity (kidney transplantation, incidental abdominal condition, PD related problems excluding recent peritonitis (4weeks prior to the measurements), e.g. catheter replacement, catheter removal because of membrane failure);
3. A control group: kidney donors undergoing nephrectomy;
4. Age: 18-60 years

Exclusion Criteria

1. unstable clinical condition
2. smoking
3. recent history of peritonitis (peritonitis less than 4weeks prior to the measurement)
4. use of antioxidants

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1) The thickness of the peritoneal endothelial glycocalyx (as measured by<br /><br>Orthogonal Polarization Spectroscopy)<br /><br>2) The thickness of the sublingual endothelial glycocalyx (as measured by<br /><br>Orthogonal Polarization Spectroscopy) </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1) Parameters of peritoneal transport in PD patients<br /><br>2) Morphological alterations of the peritoneal membrane<br /><br>3) Glycocalyx constituents (hyaluronan, heparansulphate and syndecan-1) and<br /><br>their regulating enzymes (hyaluronidase and heparanase) in plasma and dialysate</p><br>