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Lyme Borreliosis and Early Cutaneous Diagnostic

Not Applicable
Completed
Conditions
Erythema Migrans
Registration Number
NCT02414789
Lead Sponsor
University Hospital, Strasbourg, France
Brief Summary

Test and evaluate a new diagnostic method (SRM-MS/MS: Selected Reaction Monitoring- Mass spectrometry) for Lyme Borreliosis on human skin biopsies. Patients included are those with the early skin manifestation (erythema migrans).

This new proteomic method will be compared to the two existing method: culture of Borrelia and PCR detection of Borrelia (DNA detection of the bacteria).

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
84
Inclusion Criteria
  • Erythema migrant as criteria EUCALB or atypical form associated to tick's sting
  • Patient having signed a consent
  • Patient affiliated to a social security scheme
Exclusion Criteria
  • Prior treatment with antibiotics
  • Erythema migrans on the face
  • Bleeding disorders
  • Anticoagulant treatment
  • Inability to give clear information (subject in an emergency situation, understanding of the topic difficulty, ...)

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Primary Outcome Measures
NameTimeMethod
Compare the sensitivity (percentage of positive tests) of the SRM / MS-MS to techniques of PCR and culture BorreliaOne year study

percentage of positive tests with all three techniques (SRM/MS-MS ; PCR ; culture)

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (7)

Centre Hospitalo-Universitaire Jean Minjoz

🇫🇷

Besancon, France

Centre Hospitalier William Morey

🇫🇷

Chalon-sur-Saône, France

Hôpital Pasteur

🇫🇷

Colmar, France

Hôpital E. Muller

🇫🇷

Mulhouse, France

Hôpital Robert Debré

🇫🇷

Reims, France

Hôpitaux Universitaires de Strasbourg

🇫🇷

Strasbourg, France

Hôpital Bel Air

🇫🇷

Thionville, France

Centre Hospitalo-Universitaire Jean Minjoz
🇫🇷Besancon, France

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