Pre-Sleep Creatine Enhances Anaerobic Power in Recreationally Active Females

Not Applicable

Recruiting

• Conditions: Anaerobic Performance; Sports Nutrition; Exercise Recovery; Muscle Fatigue; Creatine Supplementation; Recreational Physical Activity; Female Athletes

• Registration Number: NCT06937190
• Lead Sponsor: Ankara Yildirim Beyazıt University

Brief Summary

This study is a randomized, double-blind, placebo-controlled trial designed to examine the effects of pre-sleep creatine monohydrate supplementation on anaerobic performance and muscle damage markers in recreationally active females. Participants will be randomly assigned to receive either 5 grams of creatine monohydrate or a placebo 30 minutes before sleep for seven consecutive days. Anaerobic performance will be assessed using the Wingate Anaerobic Test, evaluating peak and average power output and fatigue index. Blood samples will be collected before and after the supplementation period to analyze markers of muscle damage, including creatine kinase and lactate dehydrogenase. This study aims to provide insight into the efficacy of short-term, time-specific creatine supplementation for enhancing performance and recovery.

Detailed Description

Not available

Study Timeline

• Status Verified: 2025-04
• Study Start: 2025-04-01
• Study First Submitted: 2025-04-15
• Study First Submitted QC: 2025-04-15
• Last Update Submitted: 2025-04-15
• Study First Posted: 2025-04-22
• Last Update Posted: 2025-04-22
• Primary Completion: 2025-05-10
• Study Completion: 2025-07-10

Recruitment & Eligibility

• Status: RECRUITING
• Sex: Female
• Target Recruitment: 24

Inclusion Criteria

Female, aged 18 to 25 years

Recreationally active (engaging in structured resistance training 3-4 times per week for the past 6 months)

Regular menstrual cycles (used for scheduling purposes)

Able and willing to provide informed consent

Agreement to maintain normal diet and training routines throughout the study

Exclusion Criteria

History of musculoskeletal injury affecting lower or upper limbs within the past 6 months

Known allergy or intolerance to creatine or maltodextrin

Use of performance-enhancing substances or supplements within the last 30 days

Use of recovery modalities such as massage, cryotherapy, or compression garments during the study period

Current use of anti-inflammatory medication

Pregnancy or breastfeeding

Inability to comply with study protocol or scheduled assessments

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Average Power Output (Watts) - 30-Second Wingate Test	Baseline and post-intervention (Day 0 and Day 7)	Average anaerobic power output will be calculated across the 30-second Wingate cycling test.

Secondary Outcome Measures

Name	Time	Method
Peak Power Output (Watts) - Wingate Test	Baseline and post-intervention (Day 0 and Day 7)	Peak power output during the initial 5 seconds of the 30-second Wingate cycling test.
Fatigue Index (%) - Wingate Test	Baseline and post-intervention (Day 0 and Day 7)	Calculated as the percent drop in power from peak to minimum power output during the 30-second Wingate test, indicating fatigue rate.
Serum Creatine Kinase (CK) Activity	Baseline and 24 hours post Wingate Test (Day 0 and Day 8)	Serum creatine kinase activity will be measured as a marker of muscle damage using a spectrophotometric assay.
Serum Lactate Dehydrogenase (LDH) Activity	Baseline and 24 hours post Wingate Test (Day 0 and Day 8)	Serum lactate dehydrogenase activity will be measured as an indicator of cell membrane permeability and muscle damage using spectrophotometry.

Trial Locations

Locations (1)

Islamic Azad University, Shabestar Branch; 🇮🇷 Shabestar, East Azerbaijan, Iran, Islamic Republic of