A Two-part Study in Edoxaban-treated Healthy Subjects to Establish a Punch Biopsy Bleeding Model and to Evaluate the Effect of a 4-factor Prothrombin Complex Concentrate on Anticoagulation

Phase 1

Completed

• Conditions: Bleeding
• Interventions: Drug: 60mg edoxaban; Drug: 180mg edoxaban; Drug: 50 IU/kg Beriplex P/N; Drug: 25 IU/kg Beriplex P/N; Drug: 10 IU/kg Beriplex P/N

• Registration Number: NCT02047565
• Lead Sponsor: Daiichi Sankyo

Brief Summary

This Phase 1 study consists of 2 parts. Part 1 will be an open-label, randomized, 2 treatment, 2-way crossover study. Part 2 will be a double-blind (Sponsor unblinded), randomized, placebo controlled, sequential descending prothrombin complex concentrate dose, 2 sequence, 2 period crossover study. In both parts of the study, the assessor of BD and BV will remain blinded. In Part 2 of the study, both the subject and the clinic staff involved in study conduct will be blinded (with the exception of the pharmacist or nurse who prepares the blinded individual treatments from open-label supplies). The study programmer and statistician will also be blinded to treatment assignment. The Sponsor will remain unblinded for both parts of the study.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-10
• Study First Submitted: 2013-12-02
• Study First Submitted QC: 2014-01-24
• Study First Posted: 2014-01-28
• Primary Completion: 2014-05
• Study Completion: 2014-05
• Status Verified: 2015-01
• Last Update Submitted: 2019-02-08
• Last Update Posted: 2019-02-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 110

Inclusion Criteria

• Healthy subjects between 18 and 45 years of age, with a body mass index between 18 and 30 kg/m2, and weighing ≤ 110 kg.

Exclusion Criteria

• Women of childbearing potential without proper contraceptive measures and women who are pregnant or breastfeeding. Women of childbearing potential who participate in the study must agree to use proper contraceptive measures from screening through 13 weeks after the last dose of study drug.
• Subjects with history of unexplained syncope. Subjects who have prior clearance of vasovagal events may be included.
• Subjects who have used any drugs or substances known to be strong inhibitors or strong inducers of cytochrome P450 (CYP) 3A4/5 enzymes or P-glycoprotein within 28 days prior to the first dosing.
• Subjects who have used any other nonprescription drugs (including herbal supplemental), except acetaminophen (up to 3 g/day) within 14 days prior to check-in.
• Subjects with history of major bleeding, major trauma, or major surgical procedure of any type within 6 months of dosing.
• Subjects with history of peptic ulcer, gastrointestinal bleeding (including hematemesis, melena, and rectal bleeding), or bleeding from hemorrhoids.
• Subjects with history of minor bleeding episodes such as epistaxis, rectal bleeding (spots of blood on toilet paper), and gingival bleeding within 3 months before the first dose.
• Subjects who have any family history, suspected or documented, of coagulopathy.
• Subjects who have participated in a previous edoxaban study within 6 months prior to the first dose.
• Subjects who used anticoagulants (eg, warfarin, low molecular weight heparin), antiplatelet agents (eg, clopidogrel), non-steroidal anti-inflammatory drugs, and/or acetylsalicylic acid 30 days prior to punch biopsy or who expect to use these during the study.
• Subjects with hemoglobin levels below 12 g/dL (men) or 11 g/dL (women) at screening.
• Subjects with creatinine clearance ≤ 80 mL/min (based on the Cockcroft-Gault equation).
• Subjects who are considered inappropriate for the punch biopsy procedure based on inability to visualize surface blood vessels, and history or likelihood of forming keloid scars.
• Subjects with known heparin-induced thrombocytopenia.
• Subjects who have a platelet count, PT, or INR outside of the normal range at baseline.
• Subjects with history or current evidence of clinically significant cardiac, hepatic, renal, pulmonary, endocrine, neurologic, infectious, gastrointestinal, hematologic, or oncologic disease as determined by screening history, physical examination, laboratory test results, or 12-lead electrocardiogram (ECG).

In addition, for Part 2:

• Subjects who are deficient in Factor V Leiden mutation.
• Subjects who are deficient in protein S, protein C, antithrombin, or factor II, or have prothrombin 20210A mutation.
• Subjects with known anaphylactic or severe systemic reactions to Beriplex P/N or any components in Beriplex P/N including heparin; FII, FVII, FIX, and FX; proteins C and S; antithrombin III; and human albumin.
• Subjects with current or history of disseminated intravascular coagulation.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Part 2 - 60mg edoxaban and 10 IU/kg Beriplex P/N	60mg edoxaban	Dose cohort 3: 60 mg edoxaban + 10 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period
Part 2 - 60mg edoxaban and 20 IU/kg Beriplex P/N	60mg edoxaban	Dose cohort 2: 60 mg edoxaban + 25 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period
Part 1 - 180mg edoxaban	180mg edoxaban	Treatment B: single oral dose of 180 mg edoxaban (3 × 60 mg tablet)
Part 1 - 60mg edoxaban	60mg edoxaban	Treatment A: single oral dose of 60 mg edoxaban (1 × 60 mg tablet)
Part 2 - 60mg edoxaban and 50 IU/kg Beriplex P/N	50 IU/kg Beriplex P/N	Dose cohort 1: 60 mg edoxaban + 50 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period
Part 2 - 60mg edoxaban and 50 IU/kg Beriplex P/N	60mg edoxaban	Dose cohort 1: 60 mg edoxaban + 50 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period
Part 2 - 60mg edoxaban and 20 IU/kg Beriplex P/N	25 IU/kg Beriplex P/N	Dose cohort 2: 60 mg edoxaban + 25 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period
Part 2 - 60mg edoxaban and 10 IU/kg Beriplex P/N	10 IU/kg Beriplex P/N	Dose cohort 3: 60 mg edoxaban + 10 IU/kg Beriplex P/N in 1 period and placebo (0.9% Sodium Chloride Injection, USP), in the other period

Primary Outcome Measures

Name	Time	Method
Bleeding volume 60mg edoxaban	Day 1	To assess the variability and effect size of bleeding volume (BV) following punch biopsy in healthy subjects administered 60 mg edoxaban
Bleeding duration 60mg edoxaban	Day 1	To assess the variability and effect size of bleeding duration (BD) following punch biopsy in healthy subjects administered 60 mg edoxaban
Bleeding volume 180mg edoxaban	Day 1	To assess the variability and effect size of bleeding volume (BV) following punch biopsy in healthy subjects administered 180 mg edoxaban
Bleeding duration 180mg edoxaban	Day 1	To assess the variability and effect size of bleeding duration (BD) following punch biopsy in healthy subjects administered 180 mg edoxaban

Secondary Outcome Measures

Name	Time	Method
Activated Partial Thromboplastin Time	Day 1	To evaluate the reversal of the effect of 60 mg edoxaban on Prothrombin Time (PT), International Normalized Ratio (INR), Activated Partial Thromboplastin Time (aPTT), and Thrombin Generation Assay (TGA) parameters by Beriplex P/N
Prothrombin Time	Day 1	To evaluate the reversal of the effect of 60 mg edoxaban on Prothrombin Time (PT), International Normalized Ratio (INR), Activated Partial Thromboplastin Time (aPTT), and Thrombin Generation Assay (TGA) parameters by Beriplex P/N
International Normalized Ratio	Day 1	To evaluate the reversal of the effect of 60 mg edoxaban on Prothrombin Time (PT), International Normalized Ratio (INR), Activated Partial Thromboplastin Time (aPTT), and Thrombin Generation Assay (TGA) parameters by Beriplex P/N
Thrombin Generation Assay	Day 1	To evaluate the reversal of the effect of 60 mg edoxaban on Prothrombin Time (PT), International Normalized Ratio (INR), Activated Partial Thromboplastin Time (aPTT), and Thrombin Generation Assay (TGA) parameters by Beriplex P/N
prothrombin fragment F1 + 2	Day 1	To evaluate the effects of Beriplex P/N following 60 mg edoxaban on the procoagulant markers D dimer and prothrombin fragment F1 + 2 (F1 + 2)
cmax of edoxaban and its active metabolite, D21-2393	Day 1	To evaluate single dose pharmacokinetics (PK) of edoxaban and its active metabolite, D21-2393
procoagulant markers D dimer	Day 1	To evaluate the effects of Beriplex P/N following 60 mg edoxaban on the procoagulant markers D dimer and prothrombin fragment F1 + 2 (F1 + 2)
AUC 0-24 of edoxaban and its active metabolite, D21-2393	Day 1	To evaluate single dose pharmacokinetics (PK) of edoxaban and its active metabolite, D21-2393
coagulation factor concentrations	Day 1	To evaluate the effects of Beriplex P/N following 60 mg edoxaban on coagulation factor concentrations
tmax of edoxaban and its active metabolite, D21-2393	Day 1	To evaluate single dose pharmacokinetics (PK) of edoxaban and its active metabolite, D21-2393

Trial Locations

Locations (1)

Quintiles; 🇺🇸 Overland Park, Kansas, United States