Do healthy people absorb cow-derived microRNAs from drinks?
Not Applicable
Completed
- Conditions
- Relevance of bovine milk exosomes as bioactive food compounds and use of exosomes for drug delivery.Not Applicable
- Registration Number
- ISRCTN16329971
- Lead Sponsor
- The Gerber Foundation
- Brief Summary
2020 Results article in https://doi.org/10.1186/s41544-019-0041-x results (added 10/06/2020) 2022 Results article in https://pubmed.ncbi.nlm.nih.gov/35063038/ (added 24/01/2022)
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- All
- Target Recruitment
- 12
Inclusion Criteria
Healthy adults
Exclusion Criteria
1. Pregnant women
2. Smokers
3. Persons with lactose and milk protein intolerance or gastrointestinal disorders
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method evels of six immune-relevant microRNA found in bovine milk exosomes in plasma using real-time quantitative polymerase chain reaction (RT-qPCR) before (0 h) and at timed intervals (3, 6,and 9 h) after a milk meal
- Secondary Outcome Measures
Name Time Method <br> 1. Secretion of inflammation-related cytokines by cultured human peripheral blood mononuclear cells (PBMCs) collected before and 6 h after milk consumption and stimulated with or without Concanavalin A (Con A) was assessed using a customized Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay<br> 2. Secretion of cytokines by PBMCs treated ex vivo with microRNA-loaded exosomes assessed using a customized Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay<br>