Influence of Endurance Exercise and Histamine Receptors on the Gene Expression in Skeletal Muscle

Not Applicable

Completed

• Conditions: Exercise; Histamine

• Registration Number: NCT05131555
• Lead Sponsor: University Ghent

Brief Summary

Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-08-16
• Study First Submitted: 2021-10-15
• Study First Submitted QC: 2021-11-11
• Study First Posted: 2021-11-23
• Primary Completion: 2021-12-06
• Study Completion: 2021-12-31
• Status Verified: 2024-04
• Last Update Submitted: 2024-04-11
• Last Update Posted: 2024-04-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 14

Inclusion Criteria

• Male and female
• 18-45 years
• not to medium physically active

Exclusion Criteria

• Smoking
• Chronic disease
• Supplement or medication intake
• Seasonal allergies

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Change in muscle transcriptome (direct response)	On each test-day: 0 minutes after the exercise training versus at rest.	RNA-sequencing
Change in muscle transcriptome (delayed response)	On each test-day: 180 minutes after the exercise training versus at rest.	RNA-sequencing
Muscle glycogen resynthesis	On each test-day: 180 minutes after the exercise training versus at rest.	Fluorometric determination of muscle glycogen levels
Plasma volume change	Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).	Plasma volume based on hemoglobin and hematocrit concentration.
Muscle glycogen depletion	On each test-day: 0 minutes after the exercise training versus at rest.	Fluorometric determination of muscle glycogen levels

Secondary Outcome Measures

Name	Time	Method
Heart rate during the exercise training	Continuously during training on each test-day.	Heart rate during the exercise training.
Blood lactate	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.	Capillary lactate concentration.
Blood glucose	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.	Capillary glucose concentration.
Blood histamine	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.	histamine concentration in blood samples.
Blood insulin	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.	insulin concentration in blood samples.
Muscle signaling pathways relevant for glucose metabolism and cardiovascular health	On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.	Phosphorylation status of proteins assessed by Western Blotting

Trial Locations

Locations (1)

Department of movement and sports sciences, Ghent University, Belgium; 🇧🇪 Ghent, Oost-Vlaanderen, Belgium