B cell signalling differences in acute cellular rejection after lung transplantation (HAMLET)
Completed
- Conditions
- afstoting long transplantaathost-versus-grafttransplant rejection
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
In order to be eligible to participate in this study, a subject must meet all
of the following criteria:
1. Patients (18-70 years) who underwent uni- or bilateral lung transplantation.
AND
2. Written informed consent.
AND
3. One or more episodes of acute cellular rejection within the first two years
after lung transplantation
OR
4. No episode of acute cellular rejection within the first two years after lung
transplantation.
Exclusion Criteria
1. Non-compliance to treatment
2. Active or treated malignancy (solid or hematological malignancies).
3. Who underwent a lung retransplantation.
4. Repetitive episodes of infection in advance of an episode of acute cellular
rejection
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>- BTK protein expression in peripheral B cells. (flow cytometry).<br /><br>- BCR signalling, measured by increased phosphorylation of pSYK in in vitro<br /><br>stimulated B cells compared to unstimulated B cells. (culture and phosphoflow) </p><br>
- Secondary Outcome Measures
Name Time Method <p>- To assess the T cell phenotype and activation<br /><br>- To assess the NK cell phenotype and activation<br /><br>- To measure the cytokine production by T cells<br /><br>- To determine Nuclear Factor kappa-B (NFkB) signaling in primary T cell<br /><br>subsets by means of flow cytometry.<br /><br>- Correlation of the findings in previously obtained peripheral lung<br /><br>biopsies; in order to<br /><br>clinico-pathologically correlate this with the findings of the immune cell<br /><br>subsets<br /><br>by means of immunohistochemistry (IHC).</p><br>