Adipose-tissue Derived Stem Cells: Characterization of Adipose-tissue Derived Stem Cells in Flaps Versus Liposuction
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Aarhus
- Enrollment
- 20
- Primary Endpoint
- Stem cell yield
Study Overview
Brief Summary
The aim of the study is to evaluate a physiological concentration of adipose tissue-derived stem cells (ASC) in excised fat versus fat harvested by liposuction. Participants will act as their own control as both extraction methods are performed on each participant.
Detailed Description
Adipose tissue-derived stem cells are of great interest due to their relevance in regenerative medicine and tissue engineering. A reason, being their capacity for multipotent differentiation, tissue repair and immune modulation.
To evaluate a physiological concentration level of ASCs in human fat we investigate two extraction methods of fat, excision and liposuction. This is done through a standardised setup with patients acting their own control. The study is carried out in accordance with the guidelines provided by the International Federation for Adipose Therapeutics and Science and International Society for Cellular Therapy.
Study Design
- Study Type
- Interventional
- Allocation
- Non Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- Female
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Eligible women - over the age of 18
- •Deemed suitable for abdominoplasty with abdomen pendens, fleur de lis, panniculectomy or belt lipectomy.
- •Able to read and understand Danish
Exclusion Criteria
- •Current smokers - patients not having paused for a minimum of four weeks prior to surgery.
- •Prior or current adjuvant chemo therapy
Arms & Interventions
Excision of fat
Control group
Liposuction of fat
The intervention examined is the extraction method by liposuction.
Intervention: Liposuction (Procedure)
Outcomes
Primary Outcomes
Stem cell yield
Time Frame: Cells are cultured from baseline to day 14.
Stem cell yield measured by colony forming units and number of colonies \>50 cells are counted.
Stem cell yield
Time Frame: At baseline right after isolation.
Stem cell yield measured by antibody stained flow cytometry.
Secondary Outcomes
- Adipogenic, chondrogenic and osteogenic differentiation.(Through study completion, an average of 1 year.)
- Growth kinetics(Cultured for 7 days and counted.)