Nighttime Protein Intake on Overnight Lipolysis and Morning Fat Oxidation in Obese Men

Not Applicable

Completed

• Conditions: Obesity
• Interventions: Other: Casein Protein; Other: Non-caloric placebo

• Registration Number: NCT02211391
• Lead Sponsor: Florida State University

Brief Summary

Single macronutrients will be consumed before sleep by obese men. Overnight lipolysis will be measured with the microdialysis technique by measuring glycerol concentration and blood flow in abdominal adipose tissue. Morning fat oxidation will be measured with indirect calorimetry.

The investigators hypothesize that carbohydrate intake will blunt the overnight lipolysis rate compared to casein protein and a non-caloric placebo. The investigators also hypothesize that casein and placebo will not be significantly different from each other in terms of overnight lipolysis.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-07
• Study First Submitted: 2014-08-04
• Study First Submitted QC: 2014-08-05
• Study First Posted: 2014-08-07
• Status Verified: 2015-08
• Primary Completion: 2015-08
• Study Completion: 2015-08
• Last Update Submitted: 2015-08-10
• Last Update Posted: 2015-08-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 12

Inclusion Criteria

• Healthy, sedentary overweight or obese (BMI ≥25 kg/m^2; body fat ≥ 25% ) men

Exclusion Criteria

• Men that have been exercising for more than 2 days per week for more than 45 minutes per session (within the past 6 months), have uncontrolled hypertension (Blood Pressure (BP)>160/100 mmHg, diagnosed cardiovascular disease, stroke, diabetes, thyroid or kidney dysfunction, take cholesterol medication, or have any allergies to milk products will be excluded. Smokers will also be excluded.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Casein Protein	Casein Protein	Casein protein (30g) will be consumed as the last food/caloric beverage and within 30 minute of sleep
Non-caloric Placebo	Non-caloric placebo	The non-caloric placebo beverage will will be consumed as the last food/caloric beverage and within 30 minute of sleep

Primary Outcome Measures

Name	Time	Method
Lipolysis	Change from day 1 to day 2 to day 3 of testing	Measuring lipolysis using the microdialysis technique. Samples will be collected at various time intervals; Vial 1: 30 minute; 2.0 uL/min flow rate; Vial 2: 4 hours; 0.3 uL/min flow rate; Vial 3: Overnight; 0.3 uL/min flow rate (before taking nighttime supplement); Vial 4: Morning sample #1; 2.0 uL/min flow rate (upon waking); Vial 5: Morning sample #2; 2.0 uL/min flow rate (arrive to lab); Blood flow will be measured using the Ethanol technique

Secondary Outcome Measures

Name	Time	Method
Resting Metabolic Rate (RMR)	Change from day 1 to day 2 to day 3	RMR will be measured with indirect calorimetry using the ventilated hood technique

Trial Locations

Locations (1)

Institute of Sports Science and Medicine, Florida State University; 🇺🇸 Tallahassee, Florida, United States