The Activation of Hippo Pathway in Nasal Polyps

Not Applicable

• Conditions: Nasal Polyps

• Registration Number: NCT03151954
• Lead Sponsor: Third Affiliated Hospital, Sun Yat-Sen University

Brief Summary

Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.

Detailed Description

Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.

Study Timeline

• Study Start: 2017-04-20
• Study First Submitted: 2017-04-21
• Study First Submitted QC: 2017-05-10
• Study First Posted: 2017-05-12
• Status Verified: 2018-09
• Last Update Submitted: 2018-09-10
• Last Update Posted: 2018-09-11
• Primary Completion: 2020-07-31
• Study Completion: 2020-07-31

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Clinical diagnosis of nasal polyps or deviation of nasal septum based on CT
• Must be able to withstand surgery
• At least 18 years old
• A sufficient level of education to understand study procedures and be able to communicate with site personnel and adhere to the follow-up;
• Accepted informed consent verbally and in writing

Exclusion Criteria

• The Poor Health
• Be allergic to anesthetics

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
location of YAP in tissues	through study completion, an average of 2 years	through Immunohistochemistry
protein expression level of YAP in tissues	through study completion, an average of 2 years	through western blot

Secondary Outcome Measures

Name	Time	Method
expression level of Th1 cytokines in the tissues	through study completion, an average of 2 years	through Flow cytometry
expression level of Th17 cytokines in the tissues	through study completion, an average of 2 years	through Flow cytometry
activation of hippo-YAP in NSECs on gene aspect	through study completion, an average of 2 years	quantitive real time PCR
activation of hippo-YAP in NSECs	through study completion, an average of 2 years	Flow cytometry
activation of hippo-YAP in NSECs on protein aspect	through study completion, an average of 2 years	Western Blot

Trial Locations

Locations (1)

ThirdSunYatSen; 🇨🇳 Guangzhou, Guangdong, China