The cellular and molecular interaction between tumor cells and platelets - role in diagnosis and tumor metastasis.
- Conditions
- cancer of the intestinal tractgastrointestinal tumors10017990
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 60
Inclusion Criteria
Confirmed gastrointestinal tumor: esophageal, stomach or colorectal cancer.
Treatment naive.
Exclusion Criteria
Not able to give informed consent.
Treatment with anticoagulants.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>1. Tumor education of platelets from patients with GI cancers compared to<br /><br>healthy controls (mRNA profiles, activation status)<br /><br>2. LMWPTP and PTP1B expressions pattern in oesophageal and gastric cancers and<br /><br>their precursor lesions compared to healthy tissue. </p><br>
- Secondary Outcome Measures
Name Time Method <p>1. Aggregation and thrombus formation of platelets from GI patients in response<br /><br>to tumor cells in vitro will be compared between GI cancer patients and healthy<br /><br>controls<br /><br>2. Expression of activation markers on platelets from patients with GI cancer<br /><br>and healthy controls in vitro induced by tumor cells with and without<br /><br>LMWPTP/PTP1B expression (knock down cell lines).<br /><br>3. Investigation of the molecular pathways activated in tumor cells by<br /><br>activation with platelets from GI cancer patients vs healthy controls,<br /><br>including shedding of NKG2D ligands. </p><br>