The effects of protein type and added leucine on post-exercise muscle protein synthesis

Completed

• Conditions: muscle anabolism; muscle protein deposition; 10037008; 10028302

• Registration Number: NL-OMON42133
• Lead Sponsor: niversiteit Maastricht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 11 June 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 96

Inclusion Criteria

Male
• Healthy and physically active (as determined by medical and activity questionnaire)
• 20-30 years of age
• Having given informed consent

Exclusion Criteria

• Having any identified metabolic or intestinal disorders
• Tobacco use
• Aspirin use in the 4 days prior to the experimental trial
• Consumption of prescription medications or any performance enhancing agent
• Inability to endure the strenuous exercise bouts e.g. injuries
• Alcohol intake during the 48 hours prior to each of the testing days
• Currently participating or having participated in another clinical trial during the last 4 weeks prior to the beginning of this study

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study endpoint is the fractional synthetic rate (FSR) of muscle<br /><br>protein synthesis (mixed, myofibrillar, and mitochondrial proteins) from 0-6<br /><br>hours in the post-prandial period. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary endpoints include:<br /><br>• The fractional synthetic rate (FSR) of muscle protein synthesis (mixed,<br /><br>myofibrillar, and mitochondrial proteins) from 0-2 and 2-6 hours in the<br /><br>post-prandial period.<br /><br>• Plasma free phenylalanine enrichment (expressed as MPE)<br /><br>• Plasma free tyrosine enrichment (expressed as MPE)<br /><br>• Plasma total phenylalanine (expressed as µmol/L)<br /><br>• Plasma total tyrosine (expressed as µmol/L)<br /><br>• Total plasma amino acids (AAmax [µmol/L])<br /><br>• Plasma glucose (glucosemax [mmol/L])<br /><br>• Plasma insulin (insulinmax [mU/L])</p><br>