Assessment of Oxidative Stress Contents in Jordanian Pregnant Women With Preeclampsia

Completed

• Conditions: Preeclampsia (PE); Oxidative Stress

• Registration Number: NCT07119034
• Lead Sponsor: Future University in Egypt

Brief Summary

Preeclampsia, a widespread complication affecting pregnant women globally, necessitates a thorough investigation into the role of oxidative stress in its manifestation. This study aimed to assess the oxidative stress markers-malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and thioredoxin (Trx) in preeclamptic compared to healthy pregnant women

Detailed Description

Preeclampsia, a widespread complication affecting pregnant women globally, necessitates a thorough investigation into the role of oxidative stress in its manifestation. This study aimed to assess the oxidative stress markers-malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and thioredoxin (Trx) in preeclamptic compared to healthy pregnant women.

Methods: Ethical approval was obtained from Al-Ahliyya Amman University prior to starting the study. A total of 90 participants (45 preeclamptic pregnant women and 45 healthy pregnant women) were included in this study. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured by HUMAN200 auto analyzer (Germany) using a homogeneous enzymatic colorimetric assay. A kit from GenoChem world was used to measure the MDA levels via a colorimetric analysis technique Sandwich enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of Trx and 4-HNE.

Results: The results revealed significantly elevated levels of MDA and 4-HNE in preeclamptic women compared to their healthy counterparts (P\<0.001). Although Trx levels did not differ significantly (P=0.29), marked distinctions were observed in LDL-C, TC, HDL-C, systolic and diastolic blood pressure (P\<0.001). Preeclamptic women exhibited substantially higher LDL, TC, and blood pressure, and lower HDL.

Conclusion: This study highlights the correlation between elevated oxidative stress markers and an elevated risk of preeclampsia in pregnant women, which may suggest the impact of these markers in the pathogenesis of preeclampsia. These findings underscore the need for a nuanced understanding of these markers to enhance prenatal care and facilitate targeted interventions

Study Timeline

• Study Start: 2022-11-01
• Primary Completion: 2022-11-10
• Study Completion: 2023-05-01
• Status Verified: 2025-08
• Study First Submitted: 2025-08-05
• Study First Submitted QC: 2025-08-11
• Last Update Submitted: 2025-08-11
• Study First Posted: 2025-08-12
• Last Update Posted: 2025-08-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 90

Inclusion Criteria

• Inclusion criteria specified pregnant women aged 20 to 40, diagnosed with preeclampsia based on blood pressure and proteinuria

Exclusion Criteria

• applied to those with diabetes, liver or kidney diseases, cardiovascular diseases, or using specific supplements like vitamins A, C, and E.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Oxadative stress parameters	7 months	Thioredoxin and 4-Hydroxynonenal quantification, while malondialdehyde
Thioredoxin and 4-Hydroxynonenal quantification, while malondialdehyde	7 months	Thioredoxin and 4-Hydroxynonenal quantification, while malondialdehyde

Trial Locations

Locations (1)

Al-Ahliyya Amman University; 🇯🇴 Amman, Jordan