Influence of acute physical activity on the NK-cell cytotoxicity of esophageal cancer patients
Not Applicable
- Conditions
- Esophageal carcinomaC15Malignant neoplasm of oesophagus
- Registration Number
- DRKS00008531
- Lead Sponsor
- Deutsche Sporthochschule Köln, Institut für Kreislaufforschung und Sportmedizin, Abteilung für molekulare und zelluläre Sportmedizin
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting stopped after recruiting started
- Sex
- All
- Target Recruitment
- 12
Inclusion Criteria
Esophageal carcinoma, befor Operation (tumoe removal), medical innocuousness for the sport intervention
Exclusion Criteria
Age < 18 years, therapy befor operatione (chemotherapy or radiation), medical contraindications for exercise
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Primary outcome is the nk-cell-cytotoxicity. Before, after and 12 h after the single bout of exercise blood will be drawn. From this blood samples nk-cells will be isolated and used in cytotoxicity assays with the cell line K562 as target cell.
- Secondary Outcome Measures
Name Time Method Secondary edpoints are the characterization of NK-cells and the tumorinfiltration by NK-cells.<br>For the characterization blood will be drawn (befor, after and 12 h after exercise) and the NK-cells will be investigated for their subgroups (CD56bright and CD56dim) and for the degranulation marker CD107a by flow cytometry.<br>The tumor tissue will be removed and analysed for NK-cell infiltration by imunohistochemistry. Therefore the NK-cells will be stained by CD56 and CD57.