Timecourse of Immobilization Experiment

Not Applicable

Completed

• Conditions: Muscular Atrophy
• Interventions: Other: Lower limb immobilisation (leg brace)

• Registration Number: NCT02984332
• Lead Sponsor: University of Exeter

Brief Summary

The effect of unilateral lower limb immobilisation over one week on muscle atrophy

Detailed Description

This study aims to assess the effect of one week of unilateral leg immobilization on quadriceps muscle volume and muscle protein synthesis rates. Participants wore a leg brace on one leg and ambulated on crutches during the immobilization period. Thirteen healthy young males were recruited to undertake this study.

It is currently understood that unilateral leg immobilization will result in a reduction in muscle size (Berg et al., 1996, Wall et al., 2015) and muscle protein synthesis rates after a period of disuse (de Boer et al., 2015, Glover et al., 2008). Recent research in this area has shown that even short term periods of leg immobilization (i.e. \<1 week) will result in reductions in muscle size and muscle protein synthesis rates after the period of disuse (Wall et al., 2015). However we currently do not have detailed information on how muscle disuse affects muscle size and muscle protein synthesis over time during short term disuse (e.g. the first week).

The stable isotope tracer deuterium oxide (D2O) will be applied to assess cumulative muscle protein synthesis between days 0-2, 2-7 and 0-7 of the immobilization period in both the immobilized and non immobilized legs. This will be in-conjunction with bilateral vastus lateralis muscle biopsies before and after 2 and 7 days of immobilization. Furthremore, quadriceps muscle volume was assessed via MRI scans that again occured before and after 2 and 7 days of immobilization. To gain a mechanistic insight on the molecular changes during disuse a custom designed 48 well Taqman low density microarray genecard was applied to assess the transcriptional response on genes involved in muscle protein synthesis and breakdown.

Study Timeline

• Study Start: 2016-01
• Study First Submitted: 2016-12-02
• Study First Submitted QC: 2016-12-05
• Study First Posted: 2016-12-06
• Primary Completion: 2017-12
• Study Completion: 2019-10
• Status Verified: 2019-11
• Last Update Submitted: 2019-11-06
• Last Update Posted: 2019-11-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 13

Inclusion Criteria

BMI >18 and <28 no prescription medications or current illness -

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Exclusion Criteria

BMI <18 and >28 current prescription medication no history of leg injury

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Lower limb immobilisation	Lower limb immobilisation (leg brace)	All participants will undergo 7 days of unilateral leg immobilization. Participants will wear a leg brace (Donjoy X-ACT, DJO Global, USA) on one of their legs which will fix the leg at 40 degrees of flexion for the 7 days. Participants will not be allowed to remove the brace at any stage and are prohibited from bearing weight on the immobilized leg, and will ambulate on crutches throughout the week of immobilization.

Primary Outcome Measures

Name	Time	Method
Muscle size	Days 0, 2 and 7	Quadriceps muscle volume measured via MRI

Secondary Outcome Measures

Name	Time	Method
Muscle strength	Pre and post immobilization	Skeletal muscle one repetition maximum strength was assessed using standard gym equipment
Muscle protein synthesis	days 0-2, 2-7 and 0-7	The stable isotope tracer deuterium oxide will be used to assess muscle protein synthesis. In conjunction with bilateral vastus lateralis muscle biopsies before and after 2 and 7 days which allow for the calculation of muscle protein synthesis (fractional synthesis rate) between days 0-2, 2-7 and 0-7.

Trial Locations

Locations (1)

Sport & Health Sciences; 🇬🇧 Exeter, Devon, United Kingdom