Coffee for Optimal Metabolite Profile
- Conditions
- Healthy
- Interventions
- Other: Diet
- Registration Number
- NCT05757154
- Lead Sponsor
- Chalmers University of Technology
- Brief Summary
The aim of this project is to evaluate metabolite profile after consumption of three types of coffee differing in type of bean, degree of roasting and preparation method. Metabolite profiles will be evaluated after a) a single dose b) 3-day consumption. The study will be carried out as a three-way cross-over design with three different types of coffee. Wash-out periods where participants consume their habitual diet are implemented between all intervention periods. The first day of intervention (single dose) includes postprandial measurements during 13 hours.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 12
- Females and males
- 18 to 80 years of age
- Body mass index (BMI) 18.5-30.0 kg/m2
- Fasting glucose ≤ 6.1 mmol/l
- Low density lipoprotein (LDL) cholesterol ≤ 5.30 mmol/l
- Triglycerides ≤ 2.60 mmol/l
- Signed informed consent
- Food allergies or intolerances preventing consumption of any products included in the study.
- Unable to sufficiently understand written and spoken Swedish to provide written consent and understand information and instructions from the study personnel.
- Pregnant, lactating or planning a pregnancy during the study period.
- Antibiotic use for the last 3 months.
- Blood donation or participation in a clinical study with blood sampling within 30 days prior to screening visit and throughout the study.
- History of stomach or gastrointestinal conditions (Inflammatory bowel disease, Chron's disease, malabsorption, colostomy, bowel resection, gastric bypass surgery etc.)
- Previous major gastrointestinal surgery
- Have type I diabetes
- Thyroid disorder
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Coffee B Diet Coffee type B. All intervention arms are coffee. Three different types of coffee is tested. Differences in type of bean, degree of roasting and preparation method. Coffee A Diet Coffee type A. All intervention arms are coffee. Three different types of coffee is tested. Differences in type of bean, degree of roasting and preparation method. Coffee C Diet Coffe type C. All intervention arms are coffee. Three different types of coffee is tested. Differences in type of bean, degree of roasting and preparation method.
- Primary Outcome Measures
Name Time Method Plasma concentration-time profile over 24 hours (AUC) of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 24 hours Difference in plasma AUC between the different types of coffee for each biomarker candidate.
Plasma kinetic profiles of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 24 hours Tmax will be estimated.
Plasma concentrations of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 24 hours Difference in the plasma concentrations between the different types of coffee comparing before (baseline) and after single dose intervention.
Urine concentrations of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 24 hours Difference in the urine concentrations between the different types of coffee comparing before (baseline) and after single dose intervention.
- Secondary Outcome Measures
Name Time Method Gut microbiome 4 days Fecal samples will analyzed for composition of the gut microbiome, baseline compared with after 4 days of intervention products.
Urine concentrations of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 4 days Difference in the urine concentrations between the different types of coffee comparing before (baseline) and after single dose and three days intervention.
Blood concentrations of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 13 hours Evaluate new simple sampling techniques using dried blood spots.
Urine metabolites 24 hours Untargeted metabolomics will be performed using established methods for urine. Analyzed exploratorily using untargeted metabolomics to find potential biomarker panels that reflect coffee consumption. Baseline compared with after intervention products.
Plasma concentrations of key metabolites derived from chlorogenic acid, caffeine, trigonelline and diterpenes. 4 days Difference in the plasma concentrations between the different types of coffee comparing before (baseline) and after single dose and three days intervention.
Plasma metabolites 4 days Untargeted metabolomics will be performed using established methods for plasma. Analyzed exploratorily using untargeted metabolomics to find potential biomarker panels that reflect coffee consumption. Baseline compared with after intervention products.
Trial Locations
- Locations (1)
University of Gothenburg, Department of Food and Nutrition and Sport Science
🇸🇪Gothenburg, Sweden