Vitrified Ovarian Tissue in Cancer Patients: Assessing the Technique in Vietnam
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Ovarian Tissue Cryopreservation
- Sponsor
- Mỹ Đức Hospital
- Enrollment
- 43
- Locations
- 1
- Primary Endpoint
- Rate of Survivable follicle
- Last Updated
- 5 years ago
Overview
Brief Summary
Ovarian tissue cryopreservation (OTC) is a new approach on fertility preservation for young female patients with cancer. It is now used by many IVF centers in the world. However, this is the first study which OTC by vitrification is carried on and assessed the effectiveness of the technique.
Detailed Description
Ovaries from all less than 45 year-old breast cancer patients who undergo oophorectomy by laparoscopy after treated with chemotherapy will be enrolled to the study. Pieces of cortex of each patient will divide into two groups. Group 1 is fresh fragments (control group) and group 2 is vitrified group. Ovarian tissues in group 2 will be vitrified or warmed with medium and devices of Ova-kit type M (Kitazato, Japan). Cortex fragments of each group will have: * Follicle survival evaluation by Neutral red staining * Histological evaluation by Hematoxylin and Eosin staining * Evaluation relative Growth Differentiation Factor 9 (GDF-9) and Caspase-3 gene expression by real-time Polymerase chain reaction (PCR). Comparison of data collected from 2 groups will be carried on to evaluate the effectiveness of the technique before applying this technique for cancer patients in Vietnam.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Ovaries from all less than 45 year-old breast cancer patients who undergo oophorectomy by laparoscopy after treated with chemotherapy
Exclusion Criteria
- •The volume of ovaries is less than 2/3 of normal 3cm (L) x 2.5cm (H) x 1.5cm (W) ovary.
Outcomes
Primary Outcomes
Rate of Survivable follicle
Time Frame: After 4 hours stained by Neutral red
Percentage of survivable follicles per counted follicles in one fragment
Secondary Outcomes
- Rate of Healthy follicle by histological analysis(Immediately after tissue stained by Hematoxylin and Eosin)
- The relative expression ratio ( R ) of genes in follicles(Immediately after the intervention)