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A Phase 1, Open Label, Dose-escalation Study of the Safety, Pharmacokinetics and Antiretroviral Activity of 3BNC117

Phase 1
Completed
Conditions
Healthy
HIV
Interventions
Biological: 3 mg/kg, single dose IV administration of 3BNC117
Biological: 10 mg/kg, two doses IV of 3BNC117
Biological: 1 mg/kg, single dose IV administration of 3BNC117
Biological: 30 mg/kg, single dose IV administration of 3BNC117
Biological: 30 mg/kg, two doses IV of 3BNC117
Biological: 10 mg/kg, single dose IV administration of 3BNC117
Registration Number
NCT02018510
Lead Sponsor
Rockefeller University
Brief Summary

The proposed study is a first-in-man phase I study that aims to evaluate the safety, tolerability and pharmacokinetics of 3BNC117 in HIV-infected and HIV-uninfected subjects, and its antiretroviral activity in HIV-infected subjects.

Detailed Description

In preclinical studies carried out in humanized mice and non-human primates, 3BNC117 alone or in combination with other neutralizing antibodies led to protection from HIV-1 or SHIV infection and also to sustained suppression of HIV-1 plasma viremia. The aims of this protocol are to evaluate the safety, tolerability and pharmacokinetics profile of 3BNC117 in both HIV-infected and HIV-uninfected subjects,and its antiretroviral activity in HIV-infected subjects.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
49
Inclusion Criteria
  • Group 1 (HIV-uninfected):

    • Adult males and females, age 18 to 65
    • Amenable to HIV risk reduction counseling and agrees to maintaining behavior consistent with low risk of HIV exposure;
    • If a sexually active male or female, participating in sexual activity that could lead to pregnancy, agrees to use an effective method of contraception throughout the study.
  • Groups 2-5 (HIV-infected):

    • Age 18 to 65
    • HIV infection confirmed by ELISA and immunoblot
    • Groups 2A-D - on ART with HIV-1 plasma RNA levels below 100,000 copies/ml, or off ART for at least 8 weeks with HIV-1 plasma RNA level between 2,000-100,000 copies/ml by standard assays on 2 occasions at least 1 week apart;
    • Group 2E - Untreated HIV-infected (not on ART for at least 8 weeks): HIV-1 RNA plasma levels between 2,000 - 100,000 copies/ml;
    • Group 3 - Untreated HIV controllers (not on ART for at least 8 weeks): HIV-1 RNA plasma level of < 2,000 copies/ml by standard assays, on 2 occasions, at least 1 week apart, and ART-naive.
    • Group 4 - ART treated with HIV-1 RNA plasma level of > 20 copies/ml by standard assays on 2 occasions, at least 1 week apart, while on combination antiretroviral therapy;
    • Groups 5A and 5B - ART treated with HIV-1 RNA plasma level of < 20 copies/ml by standard assays on 2 occasions, at least 1 week apart, while on combination antiretroviral therapy;
    • Current CD4 cell count > 300 cells/µl
    • If sexually active male and female, participating in sexual activity that could lead to pregnancy, agrees to use an effective method of contraception throughout the study.

Exclusion criteria:

  • Group 1 (HIV-uninfected):

    • Confirmed HIV-1 or HIV-2 infection;

    • History of immunodeficiency or autoimmune disease; use of systemic corticosteroids, immunosuppressive anti-cancer, or other medications considered significant by the trial physician within the last 6 months;

    • Any clinically significant acute or chronic medical condition that in the opinion of the investigator would preclude participation;

    • Within the 12 months prior to enrollment, the volunteer has a history of sexually transmitted disease;

    • Hepatitis B (surface antigen, HbsAg) or hepatitis C (HCV antibodies);

    • Laboratory abnormalities in the parameters listed below:

      • Absolute neutrophil count ≤ 2,000
      • Hemoglobin ≤ 12 gm/dL if female; ≤ 13.5 gm/dL if male
      • Platelet count ≤ 140,000
      • ALT ≥ 1.25 x ULN
      • AST ≥ 1.25 x ULN
      • Total bilirubin ≥ 1.1 ULN
      • Creatinine ≥ 1.1 x ULN
      • Coagulation parameters (PT, PTT, INR) ≥ 1.1 x ULN
    • Pregnancy or breastfeeding;

    • Any vaccination within 14 days prior to 3BNC117 administration;

    • Receipt of any experimental HIV vaccine in the past or monoclonal antibody therapy of any kind in the past;

    • Participation in another clinical study of an investigational product currently or within past 12 weeks, or expected participation during this study.

  • Groups 2-5 (HIV-infected):

    • History of AIDS-defining illness

    • History of systemic corticosteroids, immunosuppressive anti-cancer, or other medications considered significant by the trial physician within the last 6 months;

    • Any clinically significant acute or chronic medical condition, other than HIV infection, that in the opinion of the investigator would preclude participation;

    • Hepatitis B (surface antigen, HbsAg) or hepatitis C (HCV antibodies);

    • Laboratory abnormalities in the parameters listed below:

      • Absolute neutrophil count ≤ 1,300
      • Hemoglobin ≤ 10 gm/dL
      • Platelet count ≤ 125,000
      • ALT ≥ 2.0 x ULN
      • AST ≥ 2.0 x ULN
      • Total bilirubin ≥ 1.1 ULN
      • Creatinine ≥ 1.1 x ULN
      • Coagulation parameters ≥ 1.1 x ULN;
    • Current antiretroviral regimen includes either maraviroc or enfuvirtide;

    • Pregnancy or breastfeeding;

    • Any vaccination within 14 days prior to 3BNC117 administration;

    • Receipt of any experimental HIV vaccine or monoclonal antibody therapy of any kind in the past;

    • Participation in another clinical study of an investigational product currently or within past 12 weeks, or expected participation during this study.

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Groups 1B3 mg/kg, single dose IV administration of 3BNC117HIV-uninfected individuals 3 mg/kg, single dose IV administration of 3BNC117
Group 1D10 mg/kg, two doses IV of 3BNC117HIV-uninfected individuals 10 mg/kg, two doses IV of 3BNC117
Group 1A1 mg/kg, single dose IV administration of 3BNC117HIV-uninfected individuals 1 mg/kg, single dose IV administration of 3BNC117
Group 1E30 mg/kg, single dose IV administration of 3BNC117HIV-uninfected individuals 30 mg/kg, single dose IV administration of 3BNC117
Group 1F30 mg/kg, two doses IV of 3BNC117HIV-uninfected individuals 30 mg/kg, two doses IV of 3BNC117
Group 2C10 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on or off ART 10 mg/kg, single dose IV administration of 3BNC117
Group 2D30 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on or off ART 30 mg/kg, single dose IV administration of 3BNC117
Group 2E30 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals off ART, VL 2,000-100,000 copies/ml 30 mg/kg, single dose IV administration of 3BNC117
Group 330 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals off ART, VL \< 2,000 copies/ml 30 mg/kg, single dose IV administration of 3BNC117
Group 5B30 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on ART, VL \< 20 copies/ml 30 mg/kg, single dose IV administration of 3BNC117
Group 1C10 mg/kg, single dose IV administration of 3BNC117HIV-uninfected individuals 10 mg/kg, single dose IV administration of 3BNC117
Group 5A10 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on ART, VL \< 20 copies/ml 10 mg/kg, single dose IV administration of 3BNC117
Group 430 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on ART, VL \< 100,000 copies/ml 30 mg/kg, single dose IV administration of 3BNC117
Group 2A1 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on or off ART 1 mg/kg, single dose IV administration of 3BNC117
Group 2B3 mg/kg, single dose IV administration of 3BNC117HIV-infected individuals on or off ART 3 mg/kg, single dose IV administration of 3BNC117
Primary Outcome Measures
NameTimeMethod
Safety and tolerability24 weeks

To evaluate the safety and tolerability profile of one or two intravenous infusions of 3BNC117 at 3 increasing dose levels in HIV-infected and HIV-uninfected individuals.

Secondary Outcome Measures
NameTimeMethod
Pharmacokinetic profile24 hours post infusion

To determine the pharmacokinetic profile of one or two intravenous infusions of 3BNC117 in HIV-uninfected and HIV-infected subjects.

Trial Locations

Locations (4)

Brigham and Women's Hospital

🇺🇸

Boston, Massachusetts, United States

University of Cologne

🇩🇪

Cologne, Germany

The Rockefeller University

🇺🇸

New York, New York, United States

Weill Cornell Medical Center

🇺🇸

New York, New York, United States

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