Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia
- Conditions
- Male Infertility
- Interventions
- Other: Biospecimen staining
- Registration Number
- NCT01178463
- Lead Sponsor
- Hillel Yaffe Medical Center
- Brief Summary
The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Male
- Target Recruitment
- 25
Inclusion Criteria
- Azoospermic patients
Exclusion Criteria
- Patients with sperm cells
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description I. Obstructive Azoospermia Biospecimen staining - II. Non-Obstructive Azoospermia Patients Biospecimen staining -
- Primary Outcome Measures
Name Time Method SSCs were identified in both obstructive and nonobstructive azoospermia patients. One year
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Hillel Yaffe Medical Center - IVF Unit
🇮🇱Hadera, Israel