Lipids and Other Bioactive Compounds of Human Breast Milk

• Conditions: Breast Milk Collection
• Interventions: Other: Breast-milk

• Registration Number: NCT03746886
• Lead Sponsor: University of Turku

Brief Summary

The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, the normal variation of various bioactive compounds and their levels is required to be able to develop formulas better corresponding the breast milk, and ensure the optimal development of also the formula-fed babies.

Detailed Description

Not available

Study Timeline

• Study Start: 2018-11-01
• Study First Submitted: 2018-11-07
• Study First Submitted QC: 2018-11-15
• Study First Posted: 2018-11-20
• Status Verified: 2021-05
• Last Update Submitted: 2021-05-05
• Last Update Posted: 2021-05-06
• Primary Completion: 2021-12-30
• Study Completion: 2021-12-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 15

Inclusion Criteria

• breast-fed child was born full-time and is healthy.
• age of the breast-fed child is 0-6 months

Exclusion Criteria

• breast-fed child was born prematurely (gestation week <37)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Breast-feeding women	Breast-milk	A single breast-milk sample will be collected from 20 women who breast-feed their child (0-6 months old).

Primary Outcome Measures

Name	Time	Method
Relative proportions (%) of fatty acids in triacylglycerols of human milk.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Quantitation with gas chromatograph coupled with flame ionization detector in relation to external standards.
Relative proportions (%) of regioisomers within selected molecular species (acyl carbon number:number of double bonds 50:1 and 52:2) in triacylglycerols.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Negative ionization of the triacylglycerols with ammonia. Selection of the molecular weight species in the first quadruple of the MS/MS. Fragmentation of the selected ions with argon. Separation of the fragment ions in the second quadrupole of the MS/MS. Calculation of the relative proportions of regioisomers with a computer software.
Quantity (mg/ml) of lipid soluble compounds in triacylglycerols of human milk.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Gravimetric analysis of the lipids.

Trial Locations

Locations (1)

Department of Biochemistry, University of Turku; 🇫🇮 Turku, Finland