Establishment of a Method for Extraction and Quantification of Gingival Tissue Nicotine y

Recruiting

• Conditions: Smoking, Cigarette
• Interventions: Diagnostic Test: biopsie

• Registration Number: NCT05736250
• Lead Sponsor: University of Liege

Brief Summary

establishment of a method for extraction and quantification of gingival tissue nicotine in relation to conventional tobacco biomarkers: a pilot study

Detailed Description

The study aim to establish a method for extraction and quantification of gingival tissue nicotine. Additionnaly, the relationship between the nicotine tissue intoxication with others smoking indicators will be evaluated as mentionned in the following PICO question:

* P: Smoker patients

* I: Biopsie of gingival tissue

* C: Non-smoker patient

* O: nicotine dependence test (FTND), salivary cotinine, fibroblast nicotine, number of cigarette consumed per day (NCC) and the pack-year (PY)

Study Timeline

• Study Start: 2022-01-14
• Study First Submitted: 2023-01-24
• Status Verified: 2023-02
• Study First Submitted QC: 2023-02-16
• Last Update Submitted: 2023-02-16
• Study First Posted: 2023-02-21
• Last Update Posted: 2023-02-21
• Primary Completion: 2023-06
• Study Completion: 2023-06

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• non smokers
• smokers

Exclusion Criteria

• not able to have biopsie

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
TEST group	biopsie	smoker patients

Primary Outcome Measures

Name	Time	Method
nicotine	baseline	nicotine in gingival fibroblast
nicotine dependance	baseline	Fagerström test for nicotine dependence

Secondary Outcome Measures

Name	Time	Method
salivary cotinine	baseline
number of cigarette consumed	baseline
pack-year	baseline

Trial Locations

Locations (1)

Salhi; 🇧🇪 Liège, Liege, Belgium