Effect of Exercise on Appetite, Energy Intake, Butyrylcholinesterase Activity and Gut Peptides in Men With Variants of the Obesity-linked FTO rs9939609 Polymorphism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Plasma acylated ghrelin concentrations (N=24)
研究概览
简要总结
Using a database of individuals with FTO genetic data, the study aims to assess the appetite, energy intake, butyrylcholinesterase, gut hormone responses to a bout of moderate- to high intensity exercise in individuals with genetic variations in the FTO gene.
详细描述
The fat mass and obesity-associated gene (FTO) rs9939609 A allele is related to obesity, greater food intake and impaired postprandial reduction of ghrelin. Exercise acutely suppresses levels of ghrelin and appetite, yet whether the response differs in people with or without the rs9939609 A allele is unknown. This study assessed the effect of exercise on appetite, appetite-regulatory hormones and energy intake in variants of the FTO rs9939609 polymorphism.
Cohort
The investigators initially recruited 202 subjects to a database and measured FTO rs9939609 genotype. From these subjects, 12 individuals homozygous for the 'obesity-risk' rs9939609 A allele and 12 homozygous for the T allele were recruited.
A sample size of 24 was chosen based on previous data which suggested that a 34 pg/ml reduction in circulating acylated ghrelin during exercise could be detected with > 80% power with a two-tailed t-test whilst assuming a standard deviation of differences of 54 pg/ml.
Familiarisation trial
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •non-smoker, not currently dieting, weight stable for >3 months (self-reported), no personal history of cardiovascular disease, metabolic disease or dyslipidaemia, European ancestry, no psychiatric or medical condition
排除标准
- •food allergies, dislike or intolerance of study foods and drinks, irregular eating patterns, use of medication that could influence hormone concentrations
研究组 & 干预措施
Control
Experimental day where participants rest.
干预措施: Control (Other)
Exercise
Experimental day where participants complete a run.
干预措施: Exercise (Other)
结局指标
主要结局
Plasma acylated ghrelin concentrations (N=24)
时间窗: 24 hours
Measured using ELISA from venous blood samples
Subjective appetite (N=24)
时间窗: 24 hours
Measured using visual analogue scales
Plasma desacyl ghrelin concentrations (N=24)
时间窗: 24 hours
Measured using ELISA from venous blood samples
Ad-libitum energy intake (N=24)
时间窗: 24 hours
Measured at laboratory-based meals
Plasma butyrylcholinesterase activity (N=24)
时间窗: First hour of main trial (three samples).
Measured using Ellman's reagent protocol
次要结局
- Plasma total glucagon-like peptide 1 concentrations (N=24)(24 hours)
- Plasma leptin concentrations (N=24)(Baseline (fasting) sample)
- Plasma total peptide yy concentrations (N=24)(24 hours)
研究者
Professor David Stensel
Professor of Exercise Metabolism
Loughborough University