The Impact of Sleep Restriction on Gastric Emptying, Appetite and Energy Intake in Healthy Individuals
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- Difference in postprandial blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine for a breakfast meal
研究概览
简要总结
This study aims to examine if partial sleep deprivation results in alterations blood markers and subjective markers of homeostatic, and hedonic appetite, as well as provide initial data as to the impact of sleep restriction on gastric emptying.
详细描述
The proposed study will take the form of a within-subjects, randomised crossover experimental design. The data will be quantitative. The study will take place for total of approximately 5 weeks / 37 days (+ up to 6 weeks depending on their schedule: Minimum duration = 5 weeks (37 days), Maximum duration = Approximately 11 weeks (79 days).
Following a telephone screening, participants will be provided with a pre-paid envelope containing secondary screening questionnaires in the form of paper documents. Either by drop-off or post to at their place of residence or place of work. The secondary screening documents are detailed under Inclusion / Exclusion criteria and Secondary screening sub-headings. Participants will then return the filled-out inclusion / exclusion documentation if they still desire to take part in the study, either through the pre-paid envelope, or to be picked up by the research team. A member of the research team will then analyse the participants responses and inform them of their eligibility to take part, via email. Following this, participants will be invited to the lab.
Upon entering the lab, participants will be provided with an informed consent form to sign. Following this, demographic information will be collected (Approximately 45 minutes to an hour): This is detailed under the preliminary data collection sub-heading. During this visit, the participant will also be provided with an actigraphy device in order to measure habitual sleep times for one week. Following this, participants will continue to be wear the actigraphy device for a subsequent week, with participants maintaining habitual duration of time in bed, although with (Potentially) altered bedtime, with 7am wake times, in order to standardise participants to 7am wake times and eliminate any erratic sleeping behaviours leading up to the first experimental trial. Participants will also be provided with a food intake and activity diary. The following visits will take the form of experimental trials (Habitual vs 50% habitual sleep): In which the variables of interest will be tested. Namely, blood markers of appetite, self-reported appetite scores, food preference and craving, heart rate variability, gastric emptying, and energy intake. Participants will not be compensated for taking part in the study but will be reimbursed the cost of travelling to and from the lab.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Difference in postprandial blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine for a breakfast meal
时间窗: Following the ingestion of a breakfast meal at 08:15, blood will be drawn every 15 minutes until 1 hour postprandially, then every 30 minutes until 3 hours postprandially.
8 blood sample will be collected in EDTA and Heparin vacutainers in order to assess blood markers of appetite, gastrointestinal function and motivation to feed. Comparisons will be made between sleep restricted vs control conditions for the area under curve (AUC) for the 3 hour postprandial period.
Difference in postprandial blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine for a lunch-time meal
时间窗: Following the ingestion of a lunchtime meal at 12:30, blood will be drawn every 15 minutes until 1 hour postprandially, then every 30 minutes until 3 hours postprandially.
8 blood sample will be collected in EDTA and Heparin vacutainers in order to assess blood markers of appetite, gastrointestinal function and motivation to feed. Comparisons will be made between sleep restricted vs control conditions for the area under curve (AUC) for the 3 hour postprandial period.
Difference in postprandial blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine for an evening meal
时间窗: Following the ingestion of an evening meal meal at 16:45, blood will be drawn every 15 minutes until 1 hour postprandially, then every 30 minutes until 3 hours postprandially.
8 blood sample will be collected in EDTA and Heparin vacutainers in order to assess blood markers of appetite, gastrointestinal function and motivation to feed. Comparisons will be made between sleep restricted vs control conditions for the area under curve (AUC) for the 3 hour postprandial period.
Difference in diurnal blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine
时间窗: Blood samples will be drawn at 08:10, and continue to be drawn at regular intervals of 15, 30 and 60 minutes until 20:00.
27 blood samples will be collected in EDTA and Heparin vacutainers throughout the day, in order to assess blood markers of appetite, gastrointestinal function and motivation to feed. Comparisons will be made between the area under curve (AUC) for the entire diurnal period (11.45 hours), for the sleep restricted vs control conditions.
Fasting blood markers: Dopamine, Endocannabinoids, Ghrelin, Leptin, Orexin, and Peptide tyrosine tyrosine
时间窗: Comparisons in fasting blood markers will be made between sleep restricted vs control conditions. A fasted blood sample will be collected during the 5 minute period from 08:10 - 08:15
A fasting blood sample will be collected in EDTA and Heparin vacutainers in order to assess blood markers of appetite, gastrointestinal function and motivation to feed.
次要结局
- Fasting food craving: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered in a fasted state during the 5 minute period elapsed from 08:10 - 08:15. Comparisons will be made between sleep restricted vs control conditions.)
- Postprandial food craving, lunch: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 12:45 - 12:50, following the lunchtime meal. Comparisons will be made between sleep restricted vs control conditions.)
- Difference in gastric emptying of an evening meal(Every 15 mins for 2 hours following test meal. Comparisons will be made between sleep restricted and control conditions.)
- Difference in subjective feelings of appetite following a breakfast meal(Every 30 minutes up until 3 hours postprandially, following the ingestion of the test meal at 08:15.)
- Difference in subjective feelings of appetite in a fasted state(Ratings of fasting subjective appetite will be collected during the 5 minute period elapsed from 08:10 - 08:15. Comparisons will be made between sleep restricted vs control conditions.)
- Difference in subjective feelings of appetite following a lunch time meal(Every 30 minutes up until 3 hours postprandially, following the ingestion of the test meal at 12:30.)
- Postprandial food craving, breakfast: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 08:30 - 08:35 following the breakfast meal. Comparisons will be made between sleep restricted vs control conditions.)
- Preprandial food craving, evening meal: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 16:40 - 16:45, prior to the evening meal. Comparisons will be made between sleep restricted vs control conditions.)
- Postprandial food craving, evening meal: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 17:00 - 17:05 following the evening meal. Comparisons will be made between sleep restricted vs control conditions.)
- Difference in subjective feelings of appetite throughout the diurnal period.(Every 30 mins throughout the 11.45 hour testing period.)
- Preprandial food craving, lunch: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 12:25 - 12:30, prior to the lunchtime meal. Comparisons will be made between sleep restricted vs control conditions.)
- Difference in subjective feelings of appetite following an evening meal(Every 30 minutes up until 3 hours postprandially, following the ingestion of the test meal at 16:45)
- Implicit liking and wanting at mid-day: The Leeds food preference questionnaire (LFPQ)(The LFPQ will be given to participants during the 15 minute period elapsed from 12:15 -12:30, immediately prior to being served the lunchtime meal.)
- Implicit liking and wanting in the evening: The Leeds food preference questionnaire (LFPQ)(The LFPQ will be given to participants during the 15 minute period elapsed from 19:45 - 20:00, immediately prior leaving the lab.)
- Heart rate variability (HRV)(Heart rate variability (HRV) data will be gathered from 21:00 on day 2 until 20:00 on day 3, using a polar H9 chest strap heart rate monitor. Differences will be examined between control (Habitual sleep) and intervention (50% of hab)
- Night-time food craving, end of trial: The Food Cravings Questionnaire-State (FCQ-S)(Scores will be gathered during the 5 minute period elapsed from 20:00 - 20:05 immediately prior to leaving the lab. Comparisons will be made between sleep restricted vs control conditions.)