Therapeutic Efficacy of Quercetin Versus Its Encapsulated Nanoparticle on Tongue Squamous Cell Carcinoma Cell Line
试验速览
- 阶段
- 2 期
- 入组人数
- 1,000,000
- 试验地点
- 1
- 主要终点
- Cytotoxicity/Cell viability.
研究概览
简要总结
Squamous cell carcinoma (SCC) is the most common oral cavity carcinoma. Conventional therapeutic modalities for oral malignancy include surgery, radiotherapy and chemotherapy alone or in combinations.The major obstacle of using current anticancer drugs is; first the non-specific tissue distribution, as these drugs are unable to distinguish between normal and cancer cells.Quercetin is a bioactive flavonoid having strong antioxidant properties. .Among all the nanomaterials, polymeric nanoparticles are of significant interest for drug delivery applications due to many unique features of nanoparticle polymers.This is the first study to investigate the anticancer effects of (Quercetin) either free or encapsulated by PLGA-PEG NPs in tongue squamous cell carcinoma (TSCC) cell line.
详细描述
Squamous cell carcinoma (SCC) is the most common oral cavity carcinoma. Conventional therapeutic modalities for oral malignancy include surgery, radiotherapy and chemotherapy alone or in combinations. The major obstacle of using current anticancer drugs is; first the non-specific tissue distribution, as these drugs are unable to distinguish between normal and cancer cells. Quercetin is a bioactive flavonoid having strong antioxidant properties. It is naturally present in a wide variety of fruits and vegetables. Among all the nanomaterials, polymeric nanoparticles are of significant interest for drug delivery applications due to many unique features of nanoparticle polymers. Polymeric nanocarriers have been fabricated from natural and synthetic polymers. Poly ethylene glycol-poly lactide-co-glycolic acid (PEG-PLGA) amphiphilic copolymer is an emergent system because it can be easily synthesized and possesses a lot of good qualities. Several previous in vitro and in vivo studies have evaluated the cytotoxic effects of quercetin and have revealed that it decreases cell viability and increases cell apoptotic rate in OSCC. However, the anti-cancer property of quercetin in tongue squamous cell carcinoma (TSCC) has not been studied yet. This is the first study to investigate the anticancer effects of (Quercetin) either free or encapsulated by PLGA-PEG NPs in tongue squamous cell carcinoma (TSCC) cell line.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Factorial
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Tongue Squamous cell carcinoma cell lines
- •Quercetin drug.
- •Quercetin-encapsulated PLGA-PEG nanoparticles (Nano-QUT)
- •Application of a Quercetin drug as chemotherapeutic drug.
- •Detection of Quercetin activity in apoptosis or cytotoxicity/cell viability.
排除标准
- •Any cancer cell line other than tongue Squamous cell carcinoma cell lines
- •Any use of Quercetin other than chemotherapy.
- •Detection of Quercetin activities other than apoptosis or cytotoxicity/cell viability
研究组 & 干预措施
Quercetin drug.
干预措施: Quercetin 3,3',4',5,6-Pentahydroxyflavone, 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one (Drug)
Quercetin-encapsulated PLGA-PEG nanoparticles
干预措施: Quercetin-encapsulated PLGA-PEG nanoparticles (Nano-QUT) (Drug)
Doxorubicin chemotherapeutic drug
干预措施: Doxorubicin chemotherapeutic drug as a positive control (Drug)
结局指标
主要结局
Cytotoxicity/Cell viability.
时间窗: within 1 week after cell line propagation
MTT assay for cellular viability: The cytotoxic impacts of the tested drugs and Nano QUT-encapsulation will be measured by MTT. The (HNO-97) cells will be cultured in 96-well plates at a density of 5 × 103 cells/well. All drugs with their described concentrations will be added to the media over tongue SCC cell lines. After a day of incubation, the dissolved MTT in PBS will be added to each well at a final concentration of 5 mg/ml, and the samples will be incubated at 37 °C for 4h. Water-insoluble dark blue formazan crystals that will be formed during MTT cleavage in actively metabolizing cells will then be dissolved in dimethyl sulfoxide (DMSO). Absorbance will be measured at A455 nm, using an ELISA microplate reader
Apoptosis.
时间窗: within 1 week after cell line propagation
Annexin V and propidium iodide (PI) stains will be used in the determination of apoptosis after treatment with the free, nano counterpart of QUT and free Dox post 24h of their incubation over the HNO-97 cell line. The apoptotic analysis will be dedicated to differentiating between early and late apoptotic cells, as well as necrotic cells. The apoptosis of the treated and untreated HNO-97 line with the proposed free, nano counterpart of Nano-QUT and Dox will be analyzed by flow cytometer apparatus
次要结局
- Gene expression of (BCL-2) .(within 1 week after cell line propagation)
- Gene expression of ( Bax gene)(within 1 week after cell line propagation)
- survival pathway (Expression of pi3k gene)(within 1 week after cell line propagation)
研究者
Hana'a Hezam Ghaleb Algadi
Ph.D. candidate at Oral &Maxillofacial Pathology Department- Faculty of Dentistry
Cairo University