Controlled and Selective Depletion of Regulatory T-cell for Cancer Treatment, Efficacy and Safety Study
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 入组人数
- 5
- 试验地点
- 2
- 主要终点
- Tumor size of hepatic and/or lung metastases, as measured with tomodensitometry (RECIST 1.1 criteria)
研究概览
简要总结
T regulatory lymphocytes were shown to be partly responsible for immune tolerance to cancer cells. In that respect these cells oppose to the mounting of an efficacious immune response needed to cure cancer. To treat advanced metastatic colorectal cancer, the investigators propose an immunotherapy consisting in autologous lymphocytes infusion depleted from T-regulatory cells, associated with a 5-day prior lymphoid-ablative chemotherapy associating cyclophosphamide (day 1 & 2) with fludarabine (day 1 to 5). To administer treatment and monitor chemotherapy safety, patients will be hospitalized for 3 weeks until complete recovery from chemotherapy. Patients will then be followed-up ambulatory for 9 months during which time they will be assessed for tumor size with computed tomography (CT) - scan (primary criteria).
详细描述
The primary goal of the proposed clinical trial is to eliminate cancer tumor using an autologous cell therapy aiming at mounting an efficient immune anti-tumor response by selectively depleting regulatory T-cell during a controlled amount of time. This strategy will be tested in patients with hepatic metastases from colorectal who are not eligible for surgery.
This is an open-label single cohort phase I-II therapeutic trial. Patients with hepatic metastases from primary colorectal cancer, not eligible to surgery and relapsing from conventional chemotherapy and/or targeted therapy, will be included.
Following patient inclusion:
- A lymphapheresis will be performed at D-15 which will be subjected to cell sorting /purification of regulatory T cells on the one hand and T lymphocytes depleted from regulatory T cells (effectors T-cells) on the other, and subsequently frozen and stored (The procedures for ex vivo regulatory T cell depletion has been validated in a previous study - AFSSAPS- TC 192) ;
- A lymphoid-ablative chemotherapy (cyclophosphamide + fludarabine) will be perform from D1 to D5,
- Autologous effector T-cells administration will be performed at D7. Efficacy will be assessed through tumor size change. Change in tumor size will be assessed with CTscans (RECIST criteria), MRIs (functional criteria following injection: DCEMRI and diffusion MRI to assess change in cellularity and tumor necrosis and morphological criteria RECIST), and sonography with contrast injection (to assess vascular microcirculation). Assessments will be done prior to lymphoid-ablation and then monthly for 9 months. Safety will be systematically assessed daily during in-patient period using the World Health Organisation - Common Toxicity Criteria (WHO-CTC).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Colon or rectal adenocarcinoma histologically proven;
- •Hepatic or lung metastasis (at least one with size >1cm on CT-scan);
- •Not eligible for surgery;
- •Prior treatment with fluoropyrimidines, CPT11, oxaliplatine and EGFR antibodies (Cetuximab ou Panitumumab) ± bevacizumab; When tumor has a mutated Kras, prior treatment with EGFR antibodies is not mandatory;
- •No local recurrence (on CT-scan, sonogram and/or colonoscopy);
- •Karnofsky index > 70 and PS 0 or 1;
- •ASA Score < 3 ;
- •Absence of chronic hepatopathy ;
- •Lab test : WBC: neutrophil> 2.0 109 / l, lymphocytes > 1.5 109 / l; creatinine < 1.5 x ULN or clearance ≥ 60 ml/min; AST et ALT< 5 x ULN, alkaline phosphatases < 3 x ULN; LDH < 3 x ULN; negative Coombs test ;
- •Signed informed consent.
排除标准
- •Peritoneal carcinosis on CT-scan, MRI or PET-scan;
- •Contra-indication to MRI;
- •Patient with known allergy to iodinated contrast agent, gadolinium or Sulfate Hexafluoron ;
- •Presence of metastasis at sites other than lung and liver;
- •Documented history of auto-immune disease and/ or progressing disease;
- •Infection at whatever site;
- •Documented history of allo- or autograft;
- •Undernutrition, BMI < 18;
- •History of other cancer < 5 years (excluding cancer in situ of the cervix and baso-cellular tumor of the skin) or progressing disease;
- •Women of child bearing age without contraception , or pregnant or breast feeding.
结局指标
主要结局
Tumor size of hepatic and/or lung metastases, as measured with tomodensitometry (RECIST 1.1 criteria)
时间窗: from Month 1 to Month 9
次要结局
- MRI : Assessment of tumor necrosis, cellularity and morphological criteria RECIST 1.1 (functional criteria following injection : DCEMRI and diffusion MRI)(Month 1 to Month 9)
- Immune cell reconstitution will be assessed through measuring rate of regulatory T-cell reconstitution,(day 7 to 28)
- Laboratory test: hepatic function, immune function, haematology(day 1 to 28)
- Sonography : assessment of vascular micro-circulation with contrast injection,(Month 1 to Month 9)
- Clinical Exam (WHO-CTC), Vital Signs, Adverse events(day 1 to 28)