Adipose-tissue Derived Stem Cells: Characterization of Adipose-tissue Derived Stem Cells in Flaps Versus Liposuction
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 主要终点
- Stem cell yield
研究概览
简要总结
The aim of the study is to evaluate a physiological concentration of adipose tissue-derived stem cells (ASC) in excised fat versus fat harvested by liposuction. Participants will act as their own control as both extraction methods are performed on each participant.
详细描述
Adipose tissue-derived stem cells are of great interest due to their relevance in regenerative medicine and tissue engineering. A reason, being their capacity for multipotent differentiation, tissue repair and immune modulation.
To evaluate a physiological concentration level of ASCs in human fat we investigate two extraction methods of fat, excision and liposuction. This is done through a standardised setup with patients acting their own control. The study is carried out in accordance with the guidelines provided by the International Federation for Adipose Therapeutics and Science and International Society for Cellular Therapy.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Eligible women - over the age of 18
- •Deemed suitable for abdominoplasty with abdomen pendens, fleur de lis, panniculectomy or belt lipectomy.
- •Able to read and understand Danish
排除标准
- •Current smokers - patients not having paused for a minimum of four weeks prior to surgery.
- •Prior or current adjuvant chemo therapy
研究组 & 干预措施
Liposuction of fat
The intervention examined is the extraction method by liposuction.
干预措施: Liposuction (Procedure)
Excision of fat
Control group
结局指标
主要结局
Stem cell yield
时间窗: Cells are cultured from baseline to day 14.
Stem cell yield measured by colony forming units and number of colonies \>50 cells are counted.
次要结局
- Adipogenic, chondrogenic and osteogenic differentiation.(Through study completion, an average of 1 year.)
- Growth kinetics(Cultured for 7 days and counted.)