PARROT: Postprandial plasma amino acid concentrations after dairy consumptio

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 10

Inclusion Criteria

• Age over 60y
• BMI 21-30 kg/m2
• Non-smoking
• Healthy as assessed by the NIZO lifestyle and health questionnaire (*Verklaring leefgewoonten en gezondheid*).
• Regular and normal Dutch eating habits as assessed by the NIZO lifestyle and health questionnaire (3 main meals per day)
• Veins suitable for cannulation (blood sampling)
• Voluntary participation
• Having given written informed consent
• Willing to comply with study procedures
• Accept use of all encoded data, including publication, and the confidential use and storage of all data for 15 years.
• Accept disclosure of the financial benefit of participation in the study to the authorities concerned

Exclusion Criteria

• Participation in any clinical trial including blood sampling and/or administration of substances up to 30 days before Day 01 of this study
• Having a history of medical or surgical events that may significantly affect the study outcome, including: Inflammatory bowel disease, hepatitis, pancreatitis, ulcers, gastrointestinal or rectal bleeding; major gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel resection; known or suspected gastrointestinal disorders, colon or GI tract cancer
• Use of the following medication: glucose lowering drugs, insulin; medication that may impact gastric empyting (e.g. gastric acid inhibitors or laxatives)
• Diagnosed with diabetes, being treated for high blood glucose or increased fasting blood glucose (>7 mmol/l in finger prick blood) as assessed during screening visit
• Use of protein supplements
• Mental status that is incompatible with the proper conduct of the study
• A self-reported reported food allergy or sensitivity to dairy ingredients
• A self-reported allergy or sensitivity to acetaminophen
• Alcohol consumption > 28 units/week and 4/day
• Reported unexplained weight loss or weight gain of > 3 kg in the month prior to pre-study screening, or intention to lose weight during the study period
• Reported slimming or medically prescribed diet
• Recent blood donation (<1 month prior to Day 01 of the study)
• Not willing or afraid to give up blood donation during the study
• Personnel of NIZO food research or Wageningen University, department of Human Nutrition, their partner and their first and second degree relatives
• Not having a general practitioner
• Not willing to accept information-transfer concerning participation in the study, or information regarding his health, like laboratory results and eventual adverse events to and from his general practitioner

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Plasma amino acid concentrations before and at 13 timepoints (up till 5 hours)<br /><br>after consumption of the dairy products </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Plasma glucose and insulin concentrations at 9 out of the 14 time points.<br /><br>Gastric emptying rate, based on 13CO2/12CO2 ratio in expired breath for up to 3<br /><br>hours after meal consumption, and expressed as half-emptying time and lag<br /><br>phase; and on postprandial acetaminophen concentrations in blood.<br /><br>Subjective rating of appetite sensations (hunger, fullness, satiety, desire to<br /><br>eat and prospective food consumption). </p><br>