The Effect of Beetroot Juice on Plasma Trimethylamine N-oxide Concentration

Not Applicable

• Conditions: Hypertension
• Interventions: Other: nitrate; Dietary Supplement: beetroot juice

• Registration Number: NCT05004935
• Lead Sponsor: Poznan University of Physical Education

Brief Summary

The primary aim of the current study is to determine the effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration.

A secondary aims: exploring the effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status, and measurement of changes in the skin nicotinamide-adenine dinucleotide reduced form (NADH) content during transient ischemia and reperfusion.

Detailed Description

In a random order subjects will consume beetroot juice (140ml), or equimolar nitrates (NaNO3). Antecubital vein blood samples will be taken at the beginning, and every hour for 4 hours following consumption.

Plasma trimethylamine N-oxide, total antioxidant status, and nitrite/nitrate concentrations will be determined.

Using Flow Mediated Skin Fluorescence skin NADH content determined during controlled ischemia triggered by total occlusion of the brachial artery by the brachial blood pressure cuff and during reperfusion after deflation of the blood pressure cuff 2 and 4 hours following consumption.

Study Timeline

• Study First Submitted: 2021-08-05
• Study First Submitted QC: 2021-08-11
• Study First Posted: 2021-08-13
• Study Start: 2021-09-01
• Status Verified: 2023-09
• Last Update Submitted: 2023-11-14
• Last Update Posted: 2023-11-15
• Primary Completion: 2023-12-01
• Study Completion: 2023-12-15

Recruitment & Eligibility

• Status: ENROLLING_BY_INVITATION
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

healthy volunteers

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
nitrates	nitrate	single intake of 800mg nitrates (NaNO3)
beetroot juice	beetroot juice	single intake of beetroot juice containing 800mg nitrates

Primary Outcome Measures

Name	Time	Method
The effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration	4 hours	Determination of plasma trimethylamine N-oxide concentration following the beetroot juice consumption using ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method.

Secondary Outcome Measures

Name	Time	Method
The effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status	4 hours	Determination of plasma total antioxidant status following the beetroot juice consumption using commercially available kit.

Trial Locations

Locations (1)

Akademia Wychowania Fizycznego; 🇵🇱 Poznan, Poland