Anesthetic Effect on Immune Cell in Patients With Cancer
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 47
- 试验地点
- 1
- 主要终点
- immune cell (NK cell and CD8+ T cell)
研究概览
简要总结
Anesthetics agents has an effect on immune response during the cancer surgery.This influence can regulatory to immune activity or cancer cell survival.
The purpose of this study is to prove the variation of immune cell activity between preoperative and postoperative period.
详细描述
The patients were allocated randomly to receive propofol or sevoflurane. Also, a total of 18ml of blood sample was obtained for total 3 times in consecutive order.
- immediate before anesthesia induction
- postoperative 1 hours
- postoperative 24 hours
Immune cells isolation from patients peripheral blood mononuclear cells. Next, immune cell were co-culture with human cancer cell line (MCF-7) for 24 hours. investigation for immune cell or cancer cell survival by flow cytometry.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Factorial
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •patient who was planned to undergo breast cancer surgery.
排除标准
- •age < 20 years old
- •history of hypersensitivity reaction in propofol or sevoflurane
- •history of previous cancer
- •patient with ongoing inflammation
研究组 & 干预措施
sevoflurane
patients under sevoflurane anesthesia
干预措施: Sevoflurane (Drug)
propofol
patients under propofol anesthesia
干预措施: propofol (Drug)
结局指标
主要结局
immune cell (NK cell and CD8+ T cell)
时间窗: up to 24 hours
Patients blood sample are collect up to 24 hours postoperatively. These blood sample are collect in EDTA tube for NK cell and CD8+ T cell isolation from peripheral blood mononuclear cells (PBMCs). Isolation for NK cell, Ab stain with CD16 and CD56. Also, isolation for CD8+ T cell, Ab stain with CD8, that are purified from PBMCs, using FACSAria according to the manufacturer's protocol. These cell are culture with MCF-7 cancer cell line for 24 hours. After 24h, the degree of apoptosis of NK cell and CD8+ T cell was determined by flow cytometry. Suspension cell is NK cell or CD8+ T cell, these cells were harvest and washed with cell staining buffer. After washing, cell resuspended with Annexin-V binding buffer and stained with FITC-Annexin-V according to the manufacturer's protocol with analysis
次要结局
- cancer cell (MCF-7) apoptosis(Baseline. postoperative 1 hours and 24 hours)
研究者
Seong-Hyop Kim
Associate Professor
Konkuk University Medical Center