Breaking Sitting and Metabolic Health in Sedentary Lean Adults

Not Applicable

Completed

• Conditions: Metabolic Health; Gut Hormones; Energy Balance
• Interventions: Behavioral: Breaking sitting; Behavioral: Prolonged sitting

• Registration Number: NCT05306613
• Lead Sponsor: National Taiwan Normal University

Brief Summary

The purpose of this project is to investigate the effects of breaking up prolonged sitting on postprandial metabolic responses, gut hormones secretion and energy balance in sedentary lean adults.

Detailed Description

Two trials are included - Prolonged sitting and Breaking sitting.

In the Prolonged sitting trial, after consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min. In the Breaking sitting trial, after consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min. Blood samples were collected in a regular pattern throughout the trials. Energy balance (e.g., energy intake and spontaneous physical activity) was assessed using food diary and an accelerometer until midnight in the trial days once participants left laboratory.

Study Timeline

• Study Start: 2020-12-30
• Study First Submitted: 2022-03-15
• Study First Submitted QC: 2022-03-23
• Study First Posted: 2022-04-01
• Primary Completion: 2023-03-01
• Study Completion: 2023-03-01
• Status Verified: 2024-04
• Last Update Submitted: 2024-04-29
• Last Update Posted: 2024-05-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 13

Inclusion Criteria

• Physical inactive males and females
• Aged between 20 and 45 years
• Lean healthy (waist circumference less than 90 cm for males and 80 cm for females)
• Weight stable for more than 3 months (no change in weight +/- 3%)
• Non-smoker
• Able to walk comfortably on a treadmill

Exclusion Criteria

• Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia
• Waist circumference > 90 cm for male and > 80 cm for female
• Taking medications that may influence lipid or carbohydrate metabolism or immune system function
• Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Breaking sitting	Breaking sitting	Participants walked regularly during the trial.
Prolonged sitting	Prolonged sitting	Participants sat on a chair during the trial.

Primary Outcome Measures

Name	Time	Method
Gut hormones responses	320 minutes	Changes in blood gut hormones responses (e.g., GLP-1, PYY and GIP concentrations measured in pmol/L) between trials

Secondary Outcome Measures

Name	Time	Method
Visual analogue scale (VAS)	320 minutes	Changes in VAS from 0-100 mm (e.g., appetite and mood, etc.) between trials
Physical activity level	600 minutes after leaving the laboratory	Changes in physical activity level (e.g., using an accelerometer to record) after leaving the laboratory until midnight
Blood pressure	320 minutes	Changes in blood pressure (systolic and diastolic blood pressure) between trials
Computerized cognitive testing	320 minutes	Changes in cognitive function (e.g., accuracy and reaction time) between trials
Energy intake	600 minutes after leaving the laboratory	Changes in energy intake (e.g, using a food diary to record) after leaving the laboratory until midnight
Lipid profile	320 minutes	Changes in blood lipid profile (e.g., TAG and cholesterol, etc.) between trials
Incremental area under curve (iAUC) for metabolic health	320 minutes	Changes in incremental area under curve (iAUC) for insulin and glucose between trials

Trial Locations

Locations (1)

National Taiwan Normal University; 🇨🇳 Taipei, Taiwan