The impact of habitual dietary protein intake on the anabolic response in elderly me
Completed
- Conditions
- Age-related muscle losssarcopenia1003700810028302
- Registration Number
- NL-OMON38469
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
* Healthy males
* Age between 55 and 75 y
* BMI between 18.5 and 30 kg/m2
Exclusion Criteria
* Lactose intolerance
* Smoking and alcohol abuse
* Diabetes
* Diagnosed GI tract diseases
* Arthritic conditions
* A history of neuromuscular problems
* Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
* Use of anticoagulants
* Participation in exercise program
* Hypertension, high blood pressure that is above 140/90 mmHg.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main study endpoint is muscle protein synthesis (MPS) rates. In order to<br /><br>determine the MPS, the following parameters will be measured:<br /><br>* Muscle protein-bound L-[D5]-phenylalanine enrichment (expressed as MPE)<br /><br>* Muscle free (intracellular) L-[D5]-phenylalanine enrichment (expressed as MPE)<br /><br>* Plasma L-[D5]-phenylalanine enrichment (expressed as MPE)</p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary endpoints include protein digestion and amino acid absorption<br /><br>kinetics and whole-body protein synthesis, breakdown, oxidation, and net<br /><br>balance. Therefore, the following parameters will be measured:<br /><br>* Plasma phenylalanine, tyrosine, and leucine concentration (expressed as *mol/<br /><br>L)<br /><br>* Plasma enrichments (in MPE) of:<br /><br>o L-[1-13C]-phenylalanine<br /><br>o L-[D5]-phenylalanine<br /><br>o L-[1-13C]-tyrosine<br /><br>o L-[D4]-tyrosine<br /><br>o L-[D2]-tyrosine<br /><br>o L-[1-13C]-leucine<br /><br>o L-[1-13C]-alpha-KIC<br /><br>Other study parameters include plasma glucose and insulin concentrations, age,<br /><br>body weight, body length, BMI, body composition, blood pressure, and leg<br /><br>volume. </p><br>