The Pathophysiology of Disrupted Endothelial Barrier Integrity in septic shock
- Conditions
- Septic shock
- Registration Number
- NL-OMON21518
- Lead Sponsor
- Amsterdam UMC, location AMC
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
=18 years old
-Quick SOFA score =2 with suspicion of infection
-MAP < 65 mmHg and lactate > 2 mmol/L despite volume resuscitation, requiring vasopressors
-Arterial catheter placement as part of standard care
-Inclusion within 12 hours after arriving on the IC
Exclusion Criteria
-Absence of informed consent
-Major burns = 18%
-No arterial catheter placement = 12 hours after arriving on the ICU
-Immunosuppressive treatment unrelated to sepsis (recent chemotherapy, chronic use of systemic steroids, methotrexate, tacrolimus, cellcept, ciclosporin, anti-TNF-a antibodies)
-HIV infection
-Pregnancy or breast feeding
-Transfer from another hospital
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Change in transendothelial electrical resistance (TER) which will be measured using an Electric Cell-substrate Impedance Sensing device(ECIS)
- Secondary Outcome Measures
Name Time Method - Change in endothelial permeability as assessed by a transwell system<br>- Markers of endothelial permeability and activation such as (ICAM, VCAM, occludin and PECAM)<br>- Markers of inflammation (IL-1, TNF, IL-6, IL-8 and vWF antigen level)