Accelerating Post-exercise Muscle Glycogen Resynthesis in Humans: Impact of Combined Nutrient Intake and Intermittent Pneumatic Compression or Heat Therapy

Not Applicable

Completed

• Conditions: Muscle Soreness; Glycogen Depletion; Muscle Recovery
• Interventions: Device: Heat therapy; Device: Compression sleeve; Other: Glycogen-depleting exercise session; Other: Nutrient Intake (beverage); Procedure: Skeletal muscle biopsy

• Registration Number: NCT04371237
• Lead Sponsor: PepsiCo Global R&D

Brief Summary

To determine if modalities designed to improve blood flow combined with post-exercise nutrient intake will improve replenishment of muscle glycogen better than nutrient intake alone. A secondary objective is to compare the effectiveness of heat therapy and intermittent pneumatic compression on glycogen replenishment.

Detailed Description

Not available

Study Timeline

• Study Start: 2018-09-19
• Primary Completion: 2019-12-21
• Study Completion: 2019-12-21
• Study First Submitted: 2020-04-29
• Study First Submitted QC: 2020-04-29
• Study First Posted: 2020-05-01
• Status Verified: 2021-03
• Last Update Submitted: 2021-03-08
• Last Update Posted: 2021-03-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 26

Inclusion Criteria

1. Subjects will be male and female participants in good health
2. 18-35 years of age
3. Currently performing cardiovascular or intermittent exercise at a moderate to high intensity at least 5 days per week for 60 minutes or longer per day.
4. Understanding of the procedures to be undertaken as part of the study
5. Willingness to participate in exercise testing and follow instructions provided by the experimenter
6. Informed, voluntary, written consent to participate in the study

Exclusion Criteria

1. History of deep vein thrombosis
2. Obesity (BMI > 30 kg/m2)
3. Hypertension (resting SBP > 140/90 mmHg)
4. Smoking
5. Pregnant, planning to become pregnant, or lactating
6. Supplements containing antioxidants including vitamin C and E and N-acetyl cysteine
7. Any medication
8. Lactose intolerance
9. Participation in another study within the previous 30 days or in a PepsiCo funded study within the past 6 months.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Heat therapy	Heat therapy	Custom water-circulating garment on leg
Intermittent pneumatic compression	Nutrient Intake (beverage)	IPC sleeve device on leg
Heat therapy	Glycogen-depleting exercise session	Custom water-circulating garment on leg
Heat therapy	Nutrient Intake (beverage)	Custom water-circulating garment on leg
Heat therapy	Skeletal muscle biopsy	Custom water-circulating garment on leg
Intermittent pneumatic compression	Compression sleeve	IPC sleeve device on leg
Intermittent pneumatic compression	Glycogen-depleting exercise session	IPC sleeve device on leg
Intermittent pneumatic compression	Skeletal muscle biopsy	IPC sleeve device on leg

Primary Outcome Measures

Name	Time	Method
Rate of glycogen resynthesis	Change from baseline to 1.5 hrs and 3.5 hrs post exercise bout. Biopsy 1 post-exercise pre nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat, pre-nutrition intake 2, biopsy 3 at 210 min. Higher rate is better.	Leg skeletal muscle glycogen level assessment using assay kit
Muscle functional recovery: Skeletal muscle function	Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less fatigue is better.	Work fatigue (%) of the knee extensors measured using an isokinetic dynamometer
Muscle functional recovery: Perceived muscle soreness	Change from pre-exercise to post-exercise and prior to biopsies and treatments, and 24 hours post exercise. Less soreness is better.	Knee extensor muscle soreness on a visual analog scale (VAS) of 100 mm after stepping on and off a 40 cm (female) or 45 cm (male) box three times

Secondary Outcome Measures

Name	Time	Method
Serum Glucose	Change from post exercise at same time as biopsy 1 (Time 0), biopsy 2 (1.5 hrs) and biopsy 3 (3.5 hrs	Blood test using ELISA kit
mRNA expression of genes involved in regulation of glucose and glycogen metabolism and muscle functional recovery	Change from baseline to 1.5 hours and 3.5 hours post exercise bout. Biopsy 1 post-exercise pre- nutrient intake 1 and compression (IPC) or heat, biopsy 2 post compression or heat and pre- nutrition intake 2, biopsy 3 at 210 min. Higher expression better.	Leg skeletal muscle fiber analysis determined using RT-PCR (Real-time polymerase chain reaction)
Serum insulin	Change from post exercise at same time as biopsy 1 (Time 0), biopsy 2 (1.5 hrs) and biopsy 3 (3.5 hrs)	Blood test using ELISA kit

Trial Locations

Locations (1)

Purdue University, Department of Health & Kinesiology; 🇺🇸 West Lafayette, Indiana, United States