Estimation of salivary biomarker in various stages of pulpal diseases in oral cavity

Not Applicable

Completed

Conditions: Health Condition 1: K040- Pulpitis

Registration Number: CTRI/2023/07/055138

Lead Sponsor: Mudit Sharma

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 80

Inclusion Criteria

Inclusion criteria for group 1

1.Patients aged 18-40 years of age

2.Patients with no relevant medical history or absence of any systemic conditions

3.Non-smokers

4.OHI index of 0 and Russel’s Periodontal index (not >0.9)

Inclusion criteria for group II,III,IV

1.Patients with mandibular molars diagnosed with symptomatic reversible pulpitis, symptomatic irreversible pulpitis and pulpal necrosis respectively diagnosed according to AAE guidelines 2013

2.Patients with no relevant medical history or absence of any systemic conditions

Exclusion Criteria

1.Use of opioid analgesics or antibiotics 48 hours prior to specimen collection

2.Narcotic drug use - Habit of smoking or tobacco chewing

3.Having conditions that effects saliva flow such as xerostomia, radiotherapy, etc.

4.Having a systemic disease

5.Being pregnant or breastfeeding

6.Having an infectious disease known to be transmitted by saliva.

7.having Russel’s Periodontal index >0.9

8.Inability to understand the content of the informed consent form

Study & Design

Study Type: Observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
To compare salivary TNF-a levels in normal, reversible pulpitis, irreversible pulpitis & necrotic pulpal tissues by using sandwich ELISA techniqueTimepoint: 6-12 months for sample collection & evaluation of samples by ELISA

Secondary Outcome Measures

Name	Time	Method
To estimate salivary TNF-a levels in normal pulpal tissues (caries free individuals) <br/ ><br>Timepoint: 6-12 months for sample collection & evaluation of samples by ELISA;To estimate salivary TNF-a levels in patients diagnosed with pulpal necrosis.Timepoint: 6-12 months for sample collection & evaluation of samples by ELISA;To estimate salivary TNF-a levels in patients diagnosed with reversible pulpitisTimepoint: 6-12 months for sample collection & evaluation of samples by ELISA;To estimate salivary TNF-a levels in patients diagnosed with symptomatic irreversible pulpitisTimepoint: 6-12 months for sample collection & evaluation of samples by ELISA