Multiple Tracer Using Single Muscle Biopsy Technique in Evaluating Protein Synthesis and Break Down in Muscles of Cirrhotic Patients
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Fractional Synthesis Rate
研究概览
简要总结
To determine the rate and mechanisms of skeletal muscle protein synthesis and breakdown in cirrhotic patients by using multiple tracers and single muscle biopsies.
详细描述
The goal of this study is to identify the molecular regulatory mechanisms that result in loss of skeletal muscle in cirrhotic patients. Current strategies involve prolonged tracer infusion and multiple muscle biopsies. We will use an innovative approach to determine the rates of skeletal muscle protein synthesis and breakdown using multiple tracers administered at short intervals followed by a single muscle biopsy. These studies are likely to provide a conceptual advance in determining the cellular and molecular responses of the skeletal muscle in cirrhosis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Other
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •The diagnosis of cirrhosis based on liver biopsy and/or clinical, biochemical, and imaging criteria.
- •Patients with Child Pugh score from A5- to B7
- •Control group: Subject in control group should have serum ALT and AST within normal range.
- •Exclusion Criteria for both groups (cirrhotic and control) are:
- •kidney failure
- •pregnancy
- •recent surgery (bowel resection or Gastric bypass operation)
- •Advanced cardiac or pulmonary disease
- •patient with thyroid disease & Coagulopathy ( INR >1.4 and platelet count <80,000/ml)
排除标准
- 未提供
结局指标
主要结局
Fractional Synthesis Rate
时间窗: 4 hours
Fractional synthesis rate (FSR)for each patient is measured by the rate of tracer incorporation from the MIF pool to the bound pool.
次要结局
未报告次要终点
研究者
Srinivasan Dasarathy
Principal Investigator
The Cleveland Clinic