Mitochondrial Coupling Efficiency, Respiration and Vitamin B2 status in Untrained and Endurance-trained Young Females

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 32

Inclusion Criteria

18-28 year old female
- BMI 18.5-25 kg/m2
- VO2max = 37 mL/kg/min or VO2max = 47 mL/kg/min;
- Performed a valid VO2max test In order for the test to be considered valid two out of three of the following conditions should be met:
1) The maximal heart rate is within 10 beats of the predicted maximum (220 – age)
2) A plateau in in VO2 was reached; VO2 fails to increase with 150 mL/min, despite an increase in work load
3) Respiratory exchange ratio (RER) = 1.00 has been achieved

Exclusion Criteria

•Health concerns regarding respiratory and pulmonary diseases, for example COPD or (exercise induced) asthma
•(Known symptoms of) chronic diseases, for example cardiovascular disease and cancer;
•(Known symptoms of) metabolic diseases, for example type I or II diabetes or hyperthyroidism;
•(Known symptoms of) inborn metabolic diseases, for example glucose-6-phosphate dehydrogenase (G6PD) deficiency;
•(Known symptoms of) hematological disorders, for example anemia or disturbed red blood cell formation;
•Haemoglobin concentrations below 7.5 mmol/L;
•Regular smoker (defined as smoking >5 cigarettes per week);
•Lactating or pregnant;
•Following a veganistic lifestyle, i.e. excluding the consumption of animal or animal-derived food products. Vegetarians are allowed to participate in the study.
•Usage of hormonal contraceptive medications other than the birth control pill Microgynon 20/30 or a generic variant containing 0.02/0.03 mg ethinylestradiol and 0.10/0.15 mg levonorgestel. Examples include IUD contraceptives or contraceptive rings. The use of condoms or pessaria is allowed;
•Recent use (within four months) of supplements with suggestive training effects, for example creatine phosphate, EPO or anabolic steroid;
•Recent daily usage (within four weeks) of supplements containing vitamin B2 (including multivitamin supplements) and usage of vitamin B2 supplements during the study;
•Usage of recreational drugs, for example marihuana, amphetamines and cocaine during the study (starting after first screening day);
•Suffers from (sport) injury that hampers maximal exercise performance;
•Blood donation during the previous 2 months or during the course of study;
•Current participation in other clinical trials;
•Not subscribed to a general practitioner (GP) practice;
•Employed or undertaking a thesis or internship at the department of Human and Animal Physiology.

Study & Design

Study Type: Observational non invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The primary outcome is to study mitochondrial function in PBMCs from endurance-trained and untrained young females and link it to vitamin B2 status. <br>

Secondary Outcome Measures

Name	Time	Method
•To compare basal and exercise-stimulated mitochondrial function in PBMCs to analyse how exercise impacts mitochondrial function and whether this differs between endurance-trained and untrained young females;<br><br>•To analyse vitamin B2 status in cells, 24-hour urine and plasma in basal and exercise-stimulated endurance-trained and untrained young females to understand how exercise impacts vitamin B2 status and whether this differs between endurance-trained and untrained females;<br><br>•To characterize muscle mitochondrial capacity in endurance-trained and untrained young females using NIRS measurements to study the association between vitamin B2 status and muscle mitochondrial function; <br><br>•To investigate the mechanistic link between mitochondrial function in PBMCs and vitamin B2 status by analysing markers of mitochondrial and metabolic health in plasma, cells and 24-hour urine.<br>

Related Trials