Mitochondrial Coupling Efficiency, Respiration and Vitamin B2 status in Untrained and Endurance-trained Young Females

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 32

Inclusion Criteria

* 18-28 year old female
* BMI 18.5-25 kg/m2
* VO2max * 37 mL/kg/min or VO2max * 47 mL/kg/min

Exclusion Criteria

* Health concerns regarding respiratory and pulmonary diseases, for example
COPD or (exercise induced) asthma;
* (Known symptoms of) chronic diseases, for example cardiovascular disease and
cancer;
* (Known symptoms of) metabolic diseases, for example type I or II diabetes or
hyperthyroidism;
* (Known symptoms of) inborn metabolic diseases, for example
glucose-6-phosphate dehydrogenase (G6PD) deficiency;
* (Known symptoms of) hematological disorders, for example anemia or disturbed
red blood cell formation;
* Haemoglobin concentrations below 7.5 mmol/L;
* Regular smoker (defined as smoking >5 cigarettes per week);
* Lactating or pregnant;
* Following a veganistic lifestyle, i.e. excluding the consumption of animal or
animal-derived food products. Vegetarians are allowed to participate in the
study;
* Usage of hormonal contraceptive medication other than the birth control pill
Microgynon 20/30 or a generic variant containing 0.02/0.03 mg ethinylestradiol
and 0.10/0.15 mg levonorgestel. Examples include IUD contraceptives or
contraceptive rings. The use of condoms or pessaria is allowed;
* Recent use (within four months) of supplements with suggestive training
effects, for example creatine phosphate, EPO or anabolic steroid;
* Recent daily usage (within four weeks) of supplements containing vitamin B2
(including multivitamin supplements) and usage of vitamin B2 supplements during
the study;
* Usage of recreational drugs, for example marihuana, amphetamines and cocaine
during the study (starting after first screening day);
* Suffers from (sport) injury that hampers maximal exercise performance;
* Blood donation during the previous 2 months or during the course of study;
* Current participation in other clinical trials;
* Not subscribed to a general practice (GP) practice;
* Employed or undertaking a thesis or internship at the department of Human and
Animal Physiology.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study parameters are mitochondrial function in PBMCs measured using<br /><br>the Seahorse XFe96 analyser, expressed as mitochondrial oxygen consumption<br /><br>(pmol O2/min), and vitamin B2 status, measured using the erythrocyte<br /><br>glutathione reductase (GR) activity coefficient (EGRAC) combined with plasma<br /><br>and urine vitamin B2 and derivative analysis. </p><br>

Secondary Outcome Measures

Name	Time	Method

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