Molecular and Hormonal Responses to Diet-Induced Insulin Resistance
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 主要终点
- Change in insulin concentration
研究概览
简要总结
This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.
详细描述
Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Aged between 18 and 40 years
- •Physically active
- •Non-smoker
- •Not taking any medication known to interfere with the study
- •No history of cardiovascular disease or diabetes
- •Weight stable for the last 6 months
排除标准
- •Outside of age range
- •Sedentary
- •Taking medication known to interfere with the study
- •History of cardiovascular disease or diabetes
- •Unstable weight within last 6 months
研究组 & 干预措施
High-fat diet
Participants underwent a 7 day high-fat, high-energy diet intervention with metabolic measurements before and after
干预措施: High-fat, high-energy diet (Dietary Supplement)
结局指标
主要结局
Change in insulin concentration
时间窗: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L.
Change in markers of microvascular function
时间窗: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.
Change in glucose concentration
时间窗: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L.
Change in skeletal muscle insulin signalling
时间窗: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units.
Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers
时间窗: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min.
次要结局
- Change intramuscular triglyceride content(Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart))
研究者
Carl Hulston
Senior Lecturer; Principal Investigator
Loughborough University