Mechanisms of Anabolic Resistance in Older Humans

Recruiting

• Conditions: Aging
• Interventions: Other: Exercise

• Registration Number: NCT06617195
• Lead Sponsor: Mayo Clinic

Brief Summary

The purpose of this research is to understand how chronic inflammation affects muscle function and responses to exercise in older adults.

Detailed Description

Inflamed adipose tissue may contribute to blunted exercise response in skeletal muscle of older adults. The objective of this project is to evaluate a hypothesis that inflamed adipose secretes factors that activate inflammatory cascades in skeletal muscle, which may interfere with exercise-responsive molecular pathways and contribute to dysfunctional muscle phenotypes with aging. This project determine how adipose tissue influences skeletal muscle function and anabolic response to exercise in older adults. Young and older adults will complete studies to assess molecular response to acute exercise from protein synthesis rates, mRNA of exercise-responsive genes, and activation of signaling proteins in skeletal muscle. Adipose tissue will be assessed using a combination of non-invasive imaging and biopsy-based molecular phenotyping. The project will determine if acute exercise response is attenuated in older adults with inflamed adipose tissue phenotype.

Study Timeline

• Study First Submitted: 2024-09-13
• Study First Submitted QC: 2024-09-25
• Study First Posted: 2024-09-27
• Status Verified: 2025-01
• Study Start: 2025-01-14
• Last Update Submitted: 2025-01-17
• Last Update Posted: 2025-01-20
• Primary Completion: 2028-09-16
• Study Completion: 2030-09-16

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 120

Inclusion Criteria

Not provided

Exclusion Criteria

1. Diabetes or fasting plasma glucose 126 mg/dL
2. Body mass index (BMI) 30 kg/m2
3. Anemia (female subjects hemoglobin of <11 g/dl and male subjects hemoglobin <12 g/dl)
4. Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
5. Renal failure (serum creatinine > 1.5mg/dl)
6. Chronic active liver disease (AST>144IU/L or ALT>165IU/L)
7. Oral warfarin group medications or history of blood clotting disorders.
8. Smoking
9. Pregnancy or breastfeeding
10. Alcohol consumption greater than 2 glasses/day or other substance abuse
11. Untreated or uncontrolled hypothyroidism
12. Debilitating chronic disease (at the discretion of the investigators)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Young	Exercise	Men and women between the ages of 21-35 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).
Older	Exercise	Men and women between the ages of 70-85 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).

Primary Outcome Measures

Name	Time	Method
Anabolic response	2 months	Skeletal muscle protein synthesis rates will be measured from isotopic enrichment of muscle proteins

Trial Locations

Locations (1)

Mayo Clinic; 🇺🇸 Rochester, Minnesota, United States