Staphylokinase and ABO Group Phenotype: New Players in Staphylococcus Aureus Implant-associated Infections Development

Completed

• Conditions: Staphylococcal Infection
• Interventions: Other: Staphylococcus aureus nasal carriage infection; Other: IAI infection

• Registration Number: NCT02812446
• Lead Sponsor: Nantes University Hospital

Brief Summary

The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials \& methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods

Detailed Description

Not available

Study Timeline

• Study Start: 2012-02
• Primary Completion: 2012-09
• Study Completion: 2013-12
• Status Verified: 2016-06
• Study First Submitted: 2016-06-16
• Study First Submitted QC: 2016-06-23
• Last Update Submitted: 2016-06-23
• Study First Posted: 2016-06-24
• Last Update Posted: 2016-06-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 88

Inclusion Criteria

• infected patients with S. Aureus IAI infection
• noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward

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Exclusion Criteria

• minor

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
control group	Staphylococcus aureus nasal carriage infection	patients with Staphylococcus aureus nasal carriage
IAI patients group	IAI infection	patients with Staphylococcus aureus of implant-associated infections

Primary Outcome Measures

Name	Time	Method
IAI and NC nasal carriage isolates genetic background	september 2012	IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied
biofilm production measurement in the two groups	september 2012	The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery.; By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers.
Clonal complexes distribution	september 2012	The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery.; IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied.

Secondary Outcome Measures

Name	Time	Method
biofilm production correlation with genotype	december 2013	quantile-quantile (QQ)-plot analysis
Association of ABO group phenotype and IAI S. aureus genotype	december 2013	QQ-plots analysis
Correlation between Clonal Complexes distribution , genotype and clinical parameters	december 2013	In order to determine whether clusters of S. aureus could be associated with clinical characteristics of the patients, search of association between CCs and clinical data was performed