The application of buffy coats for histamine release assays.

• Conditions: Allergy; 10001708; IgE mediated Type 1 hypersensitivity

• Registration Number: NL-OMON30126
• Lead Sponsor: Academisch Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 18 June 2024

Recruitment & Eligibility

• Status: Pending
• Sex: Not specified
• Target Recruitment: 5

Inclusion Criteria

Healthy male buffy coat donors without IgE sensitization. Hb levels >8.5 mmol/l are required.

Exclusion Criteria

Buffy coat donors whose basophils show 1) a high spontaneous histamin release or 2) no histamin release after application of stimuli (non-responders).

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Primary study parameters:<br /><br>Titers specific IgE (as can be determined in RAST and CAP) do/do not correlate<br /><br>with histamin release when homologous allergens are applied.<br /><br>The biological activity of a purified natural allergen does/does not correlate<br /><br>with a modified/recombinant allergen.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>not available</p><br>