Angiogenesis in the endometrium of leiomyoma-related abnormal uterine bleeding.
Recruiting
- Conditions
- •Leiomyoma, fibroids, myomen.•Abnormal uteriene bleeding (AUB), hevig menstrueel bloedverlies (HMB).•Angiogenesis, angiogenese.
- Registration Number
- NL-OMON28541
- Lead Sponsor
- VU University Medical Centre
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 60
Inclusion Criteria
•Signed informed consent
•> 18 years
Exclusion Criteria
•Uterine abnormalities (except fibroids) such as endometrial polyps and adenomyosis
•deep infiltrating endometriosis,
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Histopathologic study:<br /><br>•Microvascular density (MVD), by the stained CD31/CD34.<br /><br /><br><br>In vitro study<br /><br>•Time of recovery of HUVEC monolayer confluency, which gives a level of migration index.<br /><br>•Quantity of ATP present, equivalent to the presence of metabolically active HUVEC and consequently the degree of proliferation.<br /><br>•Motility index of HUVEC, which resembles the sprouting.
- Secondary Outcome Measures
Name Time Method Histopathologic study:<br /><br>•Endothelial proliferation index, as ratio of expression of double stained CD31/CD34 and Ki-67.<br /><br>•The expression of actin (SMA positive vessels), which resemble the mature, pericyte covered vessels.<br /><br>•Difference in absolute number and percentage of pericyte-naked / immature vessels.<br>