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INDIGO-2: The Effect of High Water Intake on Glucose Regulation in Low-drinkers

Not Applicable
Completed
Conditions
Diabetes Mellitus, Type 2
Dehydration
Interventions
Other: Water Intake
Registration Number
NCT04076995
Lead Sponsor
Arizona State University
Brief Summary

Previous studies have indicated increased vasopressin due to hypertonic saline infusion impairs glucose regulation. The current study will examine the effect of low water intake on glucose regulation. No currently published study has investigated the acute effect of low water intake on glucose regulation using continuous glucose monitoring over the course of a full day. The aim of the study is to observe the effect of low water intake on glucose regulation in low drinkers. The study will study the glycemic responses to standardized meals in adults during an 11-hour period in two conditions: a) high water intake and b) low water intake. It is hypothesized that the area under the glucose curve will be greater in the low water intake trial as compared to the high water intake trial

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
7
Inclusion Criteria
  • healthy sedentary participants
  • aged 30-55 years
  • body mass 27.5-35.0 kg·m2
  • low water intake less than 1.5 L∙day-1 (male) or 1.0 L∙day-1 (female)
  • 24 h urine osmolality greater than 800 mmol/kg
Exclusion Criteria
  • diabetes HbA1c >6.5%
  • impaired liver or kidney function
  • cardiovascular disease
  • weight change of more than 3 kg in the past month
  • pregnancy
  • previous surgery on digestive tract
  • exercise >4 h/week

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Low Water IntakeWater IntakeLow water intake of 0.5 L/day in males and 0.4 L/day
High Water intakeWater IntakeHigh water intake of 3.0 L/day in males and 2.0 L/day in females
Primary Outcome Measures
NameTimeMethod
Area under the curve of Glucose at lunch2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm)
Area under the curve of Copeptin for the entire experiment11-hour window starting right before breakfast till 2 hours after dinner (7:00 am - 6:00 pm)
Area under the curve of Glucagon at lunch2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm)
Urinary glucose excretionEntire 11-hour protocol 7:00 am - 6:00 pm
Area under the curve of Glucagon at dinner2-hour window starting right before dinner (4:00 pm - 6:00 pm)
Area under the curve of Glucose at Dinner2-hour window starting right before dinner (4:00 pm - 6:00 pm)
Area Under the Curve for Plasma osmolalitythe entire 11-hour protocol 7:00 am - 6:00 pm
Area under the curve of Glucose for the entire experiment11-hour window starting right before breakfast till 2 hours after dinner (7:00 am - 6:00 pm)
Secondary Outcome Measures
NameTimeMethod
Area under the curve of Glucagon like peptide 1at lunch2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm)
Area under the curve for Thirst ratingthe entire 11-hour protocol 7:00 am - 6:00 pm

Thirst assessed by Visual analog scale

Mood assessment with a questionnairesingle measurement at baseline before the beginning of breakfast at 7:00 am and 1-hour before dinner meal at 3:00 pm

Assessed by Profile of mood state questionnaire

Area under the curve of Insulin at lunch2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm)
Area under the curve of Insulin at dinner2-hour window starting right before dinner (4:00 pm - 6:00 pm)
Area under the curve of Glucagon like peptide 1 at dinner2-hour window starting right before dinner (4:00 pm - 6:00 pm)
Area under the curve of plasma cortisolthe entire 11-hour protocol 7:00 am - 6:00 pm

Trial Locations

Locations (1)

Arizona Biomedical Collaborative

🇺🇸

Phoenix, Arizona, United States

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